INVIVO ELECTROPORATION AND STABLE TRANSFORMATION OF SKIN CELLS OF NEWBORN MICE BY PLASMID DNA

被引：311

作者：

TITOMIROV, AV

SUKHAREV, S

KISTANOVA, E

机构：

[1] Institute of Molecular Biology, U.S.S.R. Academy of Sciences, Moscow

[2] Frumkin Institute of Electrochemistry, U.S.S.R. Academy of Sciences, Moscow

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1088卷 / 01期

关键词：

TRANSFORMATION; ELECTROPORATION INVIVO; MOUSE SKIN;

D O I：

10.1016/0167-4781(91)90162-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The skin cells of newborn mice were stably transformed in vivo with the aid of electroporation. The plasmid DNA was introduced subcutaneously followed by high-voltage pulses applied to the skin pleat. NEO-resistant colonies were found in primary cell cultures obtained from the treated skin. The experiments show that in vivo electroporation can be used for the introduction of plasmid DNA into skin cells of mouse.

引用

页码：131 / 134

页数：4

共 23 条

[1]

Alwine J C, 1979, Methods Enzymol, V68, P220

[2] DIRECT INTRODUCTION OF GENES INTO RATS AND EXPRESSION OF THE GENES [J].