A GENERAL-METHOD FOR DEVELOPING IMMUNOASSAYS TO CHLOROACETANILIDE HERBICIDES

被引：27

作者：

FENG, PCC

HORTON, SR

SHARP, CR

机构：

[1] Monsanto Agricultural Company, A Unit of Monsanto Company, St. Louis, Missouri 63198

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1992年 / 40卷 / 02期

关键词：

D O I：

10.1021/jf00014a009

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Metolachlor, amidochlor, and butachlor were each conjugated to proteins via a thioether linkage. The molar ratios of haptens to protein ranged from 11 to 21 as determined by radioactivity. Polyclonal antibodies to metolachlor, amidochlor, and butachlor were obtained by immunizing rabbits with the corresponding hapten-protein conjugates. These antibodies were used in the development of sensitive inhibition ELISAs with I50 levels of less than 10 ppb. The individual ELISAs were highly specific, showing little or no cross-reactivity to other structurally similar chloroacetanilide herbicides.

引用

页码：211 / 214

页数：4

共 12 条

[1]

CAMPBELL AM, 1984, MONOCLONAL ANTIBODY

[2] DEVELOPMENT OF AN ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR ALACHLOR AND ITS APPLICATION TO THE ANALYSIS OF ENVIRONMENTAL WATER SAMPLES [J].