THE SIMIAN VARICELLA VIRUS AND VARICELLA ZOSTER VIRUS GENOMES ARE SIMILAR IN SIZE AND STRUCTURE

被引:62
作者
GRAY, WL [1 ]
PUMPHREY, CY [1 ]
RUYECHAN, WT [1 ]
FLETCHER, TM [1 ]
机构
[1] UNIFORMED SERV UNIV HLTH SCI,DEPT BIOCHEM,BETHESDA,MD 20014
关键词
D O I
10.1016/0042-6822(92)90022-H
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Simian varicella virus (SVV) DNA was purified from viral nucleocapsids and the molecular structure of the SVV genome was determined. SVV DNA was analyzed by agarose gel electrophoresis of BamHl, Bglll, EcoRl, and Pstl restriction endonuclease digests. SVV and varicella zoster virus (VZV) DNAs were demonstrated to have distinct restriction endonuclease profiles. Summation of the sizes of individual restriction endonuclease fragments indicate the size of SVV DNA is ≅121 kilobase pairs (kbp) or ≅76.8 megadaltons (Md). Electron microscopy, λ exonuclease analysis, and Southern blot DNA hybridizations were utilized to determine the molecular structure of the SVV genome and to construct restriction endonuclease maps. The results indicate that SVV DNA consists of a long component (L, ≅100 kbp) covalently linked to a short component (S, ≅20 kbp) which is composed of a unique short sequence (Us, 5.3 ± 0.7 kbp) bracketed by inverted repeat sequences (TRs and IRs, ≅7.2 kbp). The presence of 0.5 M Pstl restriction endonuclease fragments indicates that the S component may invert relative to the L component and that the genome exists in two major isomeric forms. The findings demonstrate that the SVV and VZV genomes are similar in size and structure. © 1992.
引用
收藏
页码:562 / 572
页数:11
相关论文
共 36 条
[1]  
ALLEN WP, 1974, LAB ANIM SCI, V24, P222
[2]  
Arvin A., 1987, NATURAL HIST VARICEL, P67
[3]   INTERFERON PROPHYLAXIS AGAINST SIMIAN VARICELLA IN ERYTHROCEBUS-PATAS MONKEYS [J].
ARVIN, AM ;
MARTIN, DP ;
GARD, EA ;
MERIGAN, TC .
JOURNAL OF INFECTIOUS DISEASES, 1983, 147 (01) :149-154
[4]   PROPERTIES OF THE GENOME OF EQUINE HERPESVIRUS TYPE-3 [J].
ATHERTON, SS ;
SULLIVAN, DC ;
DAUENHAUER, SA ;
RUYECHAN, WT ;
OCALLAGHAN, DJ .
VIROLOGY, 1982, 120 (01) :18-32
[5]  
AYRES JP, 1971, LAB ANIM SCI, V21, P685
[6]  
BENPORAT T, 1985, HERPESVIRUSES, V3, P105
[7]   VARICELLA-LIKE DISEASE IN MACAQUE MONKEYS [J].
BLAKELY, GA ;
LOURIE, B ;
MORTON, WG ;
EVANS, HH ;
KAUFMANN, AF .
JOURNAL OF INFECTIOUS DISEASES, 1973, 127 (06) :617-625
[8]   A VIRUS DISEASE OF CAPTIVE VERVET MONKEYS (CERCOPITHECUS AETHIOPS) CAUSED BY A NEW HERPESVIRUS [J].
CLARKSON, MJ ;
THORPE, E ;
MCCARTHY, K .
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG, 1967, 22 (1-2) :219-&
[9]   THE COMPLETE DNA-SEQUENCE OF VARICELLA-ZOSTER VIRUS [J].
DAVISON, AJ ;
SCOTT, JE .
JOURNAL OF GENERAL VIROLOGY, 1986, 67 :1759-1816
[10]   VARICELLA ZOSTER VIRUS-DNA EXISTS AS 2 ISOMERS [J].
ECKER, JR ;
HYMAN, RW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (01) :156-160