CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF THE CATALYTIC DOMAIN OF CEX, AN EXO-BETA-1,4-GLUCANASE AND BETA-1,4-XYLANASE FROM THE BACTERIUM CELLULOMONAS-FIMI

被引:30
作者
BEDARKAR, S
GILKES, NR
KILBURN, DG
KWAN, E
ROSE, DR
MILLER, RC
WARREN, RAJ
WITHERS, SG
机构
[1] UNIV BRITISH COLUMBIA, DEPT MICROBIOL, VANCOUVER V6T 1Z3, BC, CANADA
[2] UNIV BRITISH COLUMBIA, DEPT CHEM, VANCOUVER V6T 1Z1, BC, CANADA
关键词
CELLULOMONAS-FIMI; CRYSTALLIZATION; GLUCANASE; X-RAY CRYSTALLOGRAPHY; XYLANASE;
D O I
10.1016/0022-2836(92)90852-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Single crystals of the catalytic domain of Cex, an exo-β-1,4-glucanase and β-1,4-xylanase from the cellulolytic bacterium Cellulomonas fimi, have been grown in the presence of polyethylene glycol 4000 using the vapour diffusion technique. The crystals, which diffract to better than 2.0 Å resolution, belong to space group P41212 or P43212 and have cell constants: a = b = 88.21 A ̊, c = 81.10 A ̊; α = β = γ = 90 °. © 1992.
引用
收藏
页码:693 / 695
页数:3
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