QUANTITATION OF INTRATHECAL MEASLES-VIRUS IGG ANTIBODY-SYNTHESIS RATE - SUBACUTE SCLEROSING PANENCEPHALITIS AND MULTIPLE-SCLEROSIS

被引：36

作者：

CONRAD, AJ

CHIANG, EY

ANDEEN, LE

AVOLIO, C

WALKER, SM

BAUMHEFNER, RW

MIRZAYAN, R

TOURTELLOTTE, WW

机构：

[1] W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV,LOS ANGELES,CA 90073

[2] W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073

[3] UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024

[4] CHILDRENS HOSP LOS ANGELES,DEPT PEDIAT,LOS ANGELES,CA 90027

[5] UNIV SO CALIF,SCH MED,DEPT MICROBIOL,LOS ANGELES,CA 90027

[6] UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1994年 / 54卷 / 1-2期

关键词：

MEASLES; SUBACUTE SCLEROSING PANENCEPHALITIS; MULTIPLE SCLEROSIS; INTRATHECAL SYNTHESIS RATE; ANTIBODY INDEX; ENZYME-LINKED IMMUNOSORBENT ASSAY; QUANTITATION; IMMUNOGLOBULIN G;

D O I：

10.1016/0165-5728(94)90236-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A method for quantitating specific anti-viral antibodies in serum and cerebrospinal fluid (CSF) is established using enzyme-linked immunosorbent assay (ELISA). Quantitated antibody levels are used to determine intrathecal specific IgG synthesis rate for the particular antibody. Measles virus was used as a model for validating this quantitative technique: a mutated form of measles virus is a cause of subacute sclerosing panencephalitis (SSPE) and there is a possibility that measles virus is related to the cause of multiple sclerosis (MS). Matched serum and CSF samples were assayed. Concentration of anti-measles IgG was determined and intrathecal measles-specific IgG synthesis rate was calculated. For the SSPE samples, measles-specific IgG synthesis rate was elevated and comprised > 20% of the total intrathecal IgG synthesis rate; these results are consistent with the literature. The ELISA method can be performed routinely, providing a quick, simple, reproducible means of quantitating specific antibody concentrations, with sensitivity greater than 1 nanogram per milliliter. With this method, quantitation of IgG antibodies to any other viral antigen can be reliably and precisely determined.

引用

页码：99 / 108

页数：10

共 27 条

[1] INTRATHECAL PRODUCTION OF HIV ANTIBODIES IN SUSPECTED AIDS ENCEPHALOPATHY [J].