SERA OF PREECLAMPTIC WOMEN ARE NOT CYTOTOXIC TO ENDOTHELIAL-CELLS IN CULTURE

被引:20
作者
ENDRESEN, MJ [1 ]
TOSTI, E [1 ]
LORENTZEN, B [1 ]
HENRIKSEN, T [1 ]
机构
[1] AKER UNIV HOSP,CELL LAB,N-0514 OSLO,NORWAY
关键词
PREECLAMPSIA; ENDOTHELIUM; CYTOTOXICITY;
D O I
10.1016/0002-9378(95)90112-4
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The null hypothesis of this study was that sera of women with preeclampsia are not cytotoxic to endothelial cells in culture. STUDY DESIGN: Endothelial cells were incubated in the presence of sera (30% vol/vol) of either preeclamptic patients (n = 11) or normal pregnant women (n = 11). Release of chromium 51 from prelabeled cells was measured after exposure to the different sera. Viability of the cells was evaluated by trypan blue exclusion and plating efficiencies. Deoxyribonucleic acid and protein synthesis were studied by measuring incorporation of tritiated thymidine and leucine into deoxyribonucleic acid and proteins, respectively. Cell growth was determined by monitoring the number of cells per culture dish during a 5-day incubation period. RESULTS: Release of chromium 51 from endothelial cells incubated in the presence of sera from preeclamptic women was similar to controls (26.3% +/- 4.7% vs 26.7% +/- 2.5%). There was no difference in the number of trypan blue-positive cells in cultures incubated in the presence of sera from preeclamptic women and controls. Seeding the cells in either sera from preeclamptic or control women gave the same percentage bf attached cells. Similarly, preincubation of endothelial cells with either one of the two sera resulted in the same number of attached cells when they were reseeded (45% +/- 6% vs 40% +/- 15%, respectively). Incubation of endothelial cells with sera from preeclamptic or control women affected neither deoxyribonucleic acid nor protein synthesis of the endothelial cells. Furthermore, cell proliferation was similar in cultures incubated with sera from preeclamptic women and controls. CONCLUSION: No evidence was found that sera of women with preeclampsia are cytotoxic to endothelial cells in culture.
引用
收藏
页码:196 / 201
页数:6
相关论文
共 13 条
[1]   WHICH PLASMA FACTORS BRING ABOUT DISTURBANCE OF ENDOTHELIAL FUNCTION IN PREECLAMPSIA [J].
ARBOGAST, BW ;
LEEPER, SC ;
MERRICK, RD ;
OLIVE, KE ;
TAYLOR, RN .
LANCET, 1994, 343 (8893) :340-341
[2]  
BROWN MA, 1989, CURR CONCEPTS ANAEST, V17, P183
[3]  
DEGROOT CJM, 1992, 8TH P PROT PRES WORL, P279
[4]   INCREASED LIPOLYTIC-ACTIVITY AND HIGH RATIO OF FREE FATTY-ACIDS TO ALBUMIN IN SERA FROM WOMEN WITH PREECLAMPSIA LEADS TO TRIGLYCERIDE ACCUMULATION IN CULTURED ENDOTHELIAL-CELLS [J].
ENDRESEN, MJ ;
LORENTZEN, B ;
HENRIKSEN, T .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1992, 167 (02) :440-447
[5]   DECREASED PROSTACYCLIN BIOSYNTHESIS PRECEDING THE CLINICAL MANIFESTATION OF PREGNANCY-INDUCED HYPERTENSION [J].
FITZGERALD, DJ ;
ENTMAN, SS ;
MULLOY, K ;
FITZGERALD, GA .
CIRCULATION, 1987, 75 (05) :956-963
[6]   STUDY OF ANGIOTENSIN-II PRESSOR RESPONSE THROUGHOUT PRIMIGRAVID PREGNANCY [J].
GANT, NF ;
DALEY, GL ;
CHAND, S ;
WHALLEY, PJ ;
MACDONALD, PC .
JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (11) :2682-2689
[7]  
KEITH JC, 1992, 8TH P PROT PRES WORL, P218
[8]   SERA FROM PREECLAMPTIC WOMEN INCREASE THE CONTENT OF TRIGLYCERIDES AND REDUCE THE RELEASE OF PROSTACYCLIN IN CULTURED ENDOTHELIAL-CELLS [J].
LORENTZEN, B ;
ENDRESEN, MJ ;
HOVIG, T ;
HAUG, E ;
HENRIKSEN, T .
THROMBOSIS RESEARCH, 1991, 63 (03) :363-372
[9]   FASTING SERUM-FREE FATTY-ACIDS AND TRIGLYCERIDES ARE INCREASED BEFORE 20 WEEKS OF GESTATION IN WOMEN WHO LATER DEVELOP PREECLAMPSIA [J].
LORENTZEN, B ;
ENDRESEN, MJ ;
CLAUSEN, T ;
HENRIKSEN, T .
HYPERTENSION IN PREGNANCY, 1994, 13 (01) :103-109
[10]  
REDMAN CWG, 1977, LANCET, V2, P1249