ARABIDOPSIS COP1 PROTEIN SPECIFICALLY INTERACTS IN-VITRO WITH A CYTOSKELETON-ASSOCIATED PROTEIN, CIP1

被引:65
作者
MATSUI, M [1 ]
STOOP, CD [1 ]
VONARNIM, AG [1 ]
WEI, N [1 ]
DENG, XW [1 ]
机构
[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06520
关键词
SIGNAL TRANSDUCTION; LIGHT REGULATION; COILED COIL; PLANT DEVELOPMENT; SUBCELLULAR TRAFFICKING;
D O I
10.1073/pnas.92.10.4239
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Arabidopsis COP1 acts inside the nucleus to suppress photomorphogenic cellular development, and light inactivation of COP1 may involve a specific control of its nuclear activity in hypocotyls and cotyledons, but not in roots, of developing seedlings,. To understand the molecular mechanisms of COP1 action during light-mediated development, we initiated a screen for Arabidopsis cDNAs encoding proteins which interact directly with COP1 in vitro as;a step to identify the cellular components involved, We report here the isolation and characterization of a cDNA clone encoding a protein designated CIP1 (COP1-interactive protein 1). CIP1 is predominantly alpha-helical and most likely involved in coiled-coil formation, It interacts specifically with the putative coiled-coil region of COP1 in vitro, Further, CIP1 is encoded by a single gene in Arabidopsis, and its mRNA and protein levels are not regulated by light, Immunofluorescent labeling of CIP1 in Arabidopsis seedling protoplasts demonstrated that CIP1 is part of, or associated with, a cytoskeletal structure in hypocotyl. ind cotyledon cells, but not in roots, Our results are consistent with a possible role of CIP1 in mediating light control of COP1 nuclear activity by regulating its nucleocytoplasmic partitioning.
引用
收藏
页码:4239 / 4243
页数:5
相关论文
共 25 条
[1]   THE TRUNCATION THAT GENERATED THE V-CBL ONCOGENE REVEALS AN ABILITY FOR NUCLEAR TRANSPORT, DNA-BINDING AND ACUTE TRANSFORMATION [J].
BLAKE, TJ ;
HEATH, KG ;
LANGDON, WY .
EMBO JOURNAL, 1993, 12 (05) :2017-2026
[2]   A FUSCA GENE OF ARABIDOPSIS ENCODES A NOVEL PROTEIN ESSENTIAL FOR PLANT DEVELOPMENT [J].
CASTLE, LA ;
MEINKE, DW .
PLANT CELL, 1994, 6 (01) :25-41
[4]  
Chou P Y, 1978, Adv Enzymol Relat Areas Mol Biol, V47, P45
[5]   FRESH VIEW OF LIGHT SIGNAL-TRANSDUCTION IN PLANTS [J].
DENG, XW .
CELL, 1994, 76 (03) :423-426
[6]   COP1, AN ARABIDOPSIS REGULATORY GENE, ENCODES A PROTEIN WITH BOTH A ZINC-BINDING MOTIF AND A G-BETA HOMOLOGOUS DOMAIN [J].
DENG, XW ;
MATSUI, M ;
WEI, N ;
WAGNER, D ;
CHU, AM ;
FELDMANN, KA ;
QUAIL, PH .
CELL, 1992, 71 (05) :791-801
[7]   COP1 - A REGULATORY LOCUS INVOLVED IN LIGHT-CONTROLLED DEVELOPMENT AND GENE-EXPRESSION IN ARABIDOPSIS [J].
DENG, XW ;
CASPAR, T ;
QUAIL, PH .
GENES & DEVELOPMENT, 1991, 5 (07) :1172-1182
[8]   THE DTAF(II)80 SUBUNIT OF DROSOPHILA TFIID CONTAINS BETA-TRANSDUCIN REPEATS [J].
DYNLACHT, BD ;
WEINZIERL, ROJ ;
ADMON, A ;
TJIAN, R .
NATURE, 1993, 363 (6425) :176-179
[9]   COMPLETE NUCLEOTIDE-SEQUENCE AND DEDUCED POLYPEPTIDE SEQUENCE OF A NONMUSCLE MYOSIN HEAVY-CHAIN GENE FROM ACANTHAMOEBA - EVIDENCE OF A HINGE IN THE RODLIKE TAIL [J].
HAMMER, JA ;
BOWERS, B ;
PATERSON, BM ;
KORN, ED .
JOURNAL OF CELL BIOLOGY, 1987, 105 (02) :913-925
[10]   CLONING AND DEVELOPMENTAL EXPRESSION OF THE MURINE NEUROFILAMENT GENE FAMILY [J].
JULIEN, JP ;
MEYER, D ;
FLAVELL, D ;
HURST, J ;
GROSVELD, F .
MOLECULAR BRAIN RESEARCH, 1986, 1 (03) :243-250