N,N'-DIETHYL-META-TOLUAMIDE (META-DET) - ANALYSIS OF AN INSECT REPELLENT IN HUMAN URINE AND SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A procedure for monitoring m-DET In human urine and serum Is described, m-DET Is removed from the urine specimen by partitioning Into diethyl ether, but solid-phase extraction Is used to remove It from human serum. The urine and serum m-DET values ere determined by HPLC with a UV detector. The limit of detection was 0.09 µg/mL In urine and 0.09 µg/g for serum. The percent of m-DET recovered from human urine spiked between 0.50 and 8.00 µ/mL was 90 ± 5.4%. For human serum spiked between 0.25 end 10.00, the percent recovered was 96 ± 5.9%. The pooled relative standard deviations (RSD) for spiked matrices were 0.06 for urine and 0.06 for serum. © 1992, Oxford University Press. All rights reserved.