THIN-LAYER CHROMATOGRAPHY OF BILOBALIDE AND GINKGOLIDE-A, GINKGOLIDE-B, GINKGOLIDE-C, AND GINKGOLIDE-J ON SODIUM-ACETATE IMPREGNATED SILICA-GEL

被引：14

作者：

VANBEEK, TA

LELYVELD, GP

机构：

[1] Department of Organic Chemistry, Phytochemical Section, Agricultural University, Wageningen, 6703 HB

来源：

PHYTOCHEMICAL ANALYSIS | 1993年 / 4卷 / 03期

关键词：

GINKGOLIDES-A; GINKGOLIDE-B; GINKGOLIDE-C AND GINKGOLIDE-J; BILOBALIDE; THIN LAYER CHROMATOGRAPHIC SEPARATION; SODIUM ACETATE IMPREGNATED SILICA GEL; ACETIC ANHYDRIDE DETECTION;

D O I：

10.1002/pca.2800040306

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A normal phase thin layer chromatographic procedure for the separation and detection of bilobalide and ginkgolides A, B, C and J has been developed. The method is based on the impregnation of commercially available silica gel F254 plates with sodium acetate. Silica gel thus impregnated gives a good separation of all major terpene trilactones in ginkgo leaves (0.06 less-than-or-equal-to DELTAR(f) less-than-or-equal-to 0.17) with the solvent systems ethyl acetate: hexane (9: 1) (non-saturated chamber) or 100% methyl acetate (saturated chamber). The sodium acetate has two functions: it greatly improves the separation of the ginkgolide pairs G-A/G-B and G-J/G-C, and it enhances the sensitivity of the acetic anhydride spray reagent. Quantities of less than 1 mug of all four ginkgolides and bilobalide can be detected.

引用

页码：109 / 114

页数：6

共 15 条

[1]

Extract of Ginkgo biloba (EGb 761), La Presse Med., 15, pp. 1438-1604, (1986)

[2]

van Beek T.A., Scheeren H.A., Rantio T., Melger W.Ch., Lelyveld G.P., Determination of ginkgolides and bilobalide in Ginkgo biloba leaves and phytopharmaceuticals, J. Chromatogr., 543, pp. 375-387, (1991)

[3]

Braquet P., The ginkgolides: potent platelet‐activating factor antagonists isolated from Ginkgo biloba L.: chemistry, pharmacology and clinical applications, Drugs Future, 12, pp. 643-699, (1987)

[4]

Ginkgolides—Chemistry, Biology, Pharmacology and Clinical Perspectives, 1, (1988)

[5]

Ginkgolides—Chemistry, Biology, Pharmacology and Clinical Perspectives, 2, (1989)

[6]

Hasler A., Meier B., Sticher O., Ginkgo biloba—Botanische, analytische und pharmakologische Aspekte, Schweiz. Apoth. Ztg., 128, pp. 342-347, (1990)

[7]

Lobstein-Guth A., Briancon-Scheid F., Anton R., Analysis of terpenes from Ginkgo biloba L. by high‐performance liquid chromatography, Journal of Chromatography A, 267, pp. 431-438, (1983)

[8]

Okabe K., Yamada K., Yamamura S., Takada S., pp. 2201-2206, (1967)

[9]

Schilcher H., Ginkgo biloba L.—Untersuchungen zur Qualität, Wirkung, Wirksamkeit und Unbedenklichkeit, Z. Phytother., 9, pp. 119-127, (1988)

[10]

Tallevi S.G., Kurz W.G.W., Detection of ginkgolides by thin‐layer chromatography, J. Nat. Prod., 54, pp. 624-625, (1991)

← 1 2 →