Flow cytometric analysis of molt-related changes in hemocyte type in male and female Penaeus japonicus

被引:45
作者
Sequeira, T
Vilanova, M
LobodaCunha, A
Baldaia, L
AralaChaves, M
机构
[1] INST CIENCIAS BIOMED ABEL SALAZAR,IMMUNOL LAB,P-4050 OPORTO,PORTUGAL
[2] INST CIENCIAS BIOMED ABEL SALAZAR,GEN PHYSIOL LAB,P-4050 OPORTO,PORTUGAL
[3] INST CIENCIAS BIOMED ABEL SALAZAR,CELLULAR BIOL LAB,P-4050 OPORTO,PORTUGAL
关键词
D O I
10.2307/1542156
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hemocyte cell suspensions obtained from male and female Penaeus japonicus were individually analyzed by flow cytometry through forward and side light-scatter parameters. The hemocyte cell suspensions were further characterized after cell sorting. This type of cell analysis has several advantages over microscopy techniques. After staining with phenoloxidase and peroxidase, the hemocytes were classified into the three classic categories of hyaline, semigranular, and granular cells. Significant cyclic differences were detected among the molting stages in both sexes. The hyaline cell population was predominant before and soon after the molt, decreasing over the intermolt. This decrease was, however, more prolonged in females. Thus, the hyaline cell population was dominant in stages B, D0, and D1 in males and only in stages B and D1 in females. Semigranular cells became predominant in females during the D0 stage.
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收藏
页码:376 / 380
页数:5
相关论文
共 28 条
[1]   ECDYSTEROIDS IN THE SHRIMP PALAEMON-SERRATUS - RELATIONS WITH MOLT CYCLE [J].
BALDAIA, L ;
PORCHERON, P ;
COIMBRA, J ;
CASSIER, P .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1984, 55 (03) :437-443
[2]  
BALDWIN BS, 1991, AM ZOOL, V31, pA7
[4]   THE BETA-1,3-GLUCAN-BINDING PROTEIN FROM THE CRAYFISH PACIFASTACUS-LENIUSCULUS, WHEN REACTED WITH A BETA-1,3-GLUCAN, INDUCES SPREADING AND DEGRANULATION OF CRAYFISH GRANULAR CELLS [J].
BARRACCO, MA ;
DUVIC, B ;
SODERHALL, K .
CELL AND TISSUE RESEARCH, 1991, 266 (03) :491-497
[5]  
BAUCHAU A G, 1973, Crustaceana (Leiden), V24, P215, DOI 10.1163/156854073X00380
[6]  
BAUCHAU AG, 1981, INVERTEBRATE BLOOD C, V2, P386
[8]  
CROSSLEY AC, 1965, J EMBRYOL EXP MORPH, V14, P375
[9]  
DEBACKER J, 1961, ANN SOC R ZOOL BELGI, V92, P141
[10]   PROGRESSIVE DEVELOPMENT OF CIRCULATING POLYPLOID CELLS IN MYTILUS WITH HEMIC NEOPLASIA [J].
ELSTON, RA ;
DRUM, AS ;
ALLEN, SK .
DISEASES OF AQUATIC ORGANISMS, 1990, 8 (01) :51-59