MEDIATION OF 8-HYDROXY-GUANINE FORMATION IN DNA BY THIAZIN DYES PLUS LIGHT

被引：57

作者：

FLOYD, RA

WEST, MS

ENEFF, KL

SCHNEIDER, JE

机构：

[1] Molecular Toxicology Research Group, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 04期

关键词：

8-hydroxy-2′-deoxyguanosine; DNA oxidative damage; Methylene blue; Oxygen free radicals; Thiazin dyes;

D O I：

10.1016/0891-5849(90)90097-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have discovered that methylene blue plus light mediates the formation of 8-OHdG in DNA. Methylene blue is one of several thiazin dyes and we report here that the other thiazin dyes tested, in combination with white light, are effective in mediating 8-OHdG formation in DNA. The effectiveness of light plus the thiazin dyes in forming 8-OHdG in DNA were as follows: methylene blue > azure B > aure A > toluidine blue > thionin. Two other compounds tested; riboflavin and fuschin acid, in combination with light, caused formation of very little, if any, 8-OHdG in DNA. Thiazin dye mediated formation of 8-OHdG in DNA was not inhibited by the spin trap α-phenyl-t-butyl nitrone, which supports our previous observations that oxygen free radical scavengers did not inhibit methylene blue plus light mediated 8-OHdG formation in DNA. Ascobate addition to methylene blue plus DNA, in the absence of light, was ineffective in mediating 8-OHdG formation in DNA. © 1990.

引用

页码：327 / 330

页数：4

共 16 条

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