BETA-N-ACETYLHEXOSAMINADASES IN HUMAN CEREBROSPINAL-FLUID AND SERUM OF PATIENTS WITH MULTIPLE-SCLEROSIS

被引：7

作者：

DATTI, A

EMILIANI, C

CAPOCCHI, G

ORLACCHIO, A

机构：

[1] UNIV PERUGIA,DIPARTIMENTO MED SPERIMENTALE & SCI BIOCHIM,I-06100 PERUGIA,ITALY

[2] UNIV PERUGIA,CATTEDRA NEUROFISIOPATOL,I-06100 PERUGIA,ITALY

来源：

CLINICA CHIMICA ACTA | 1991年 / 200卷 / 2-3期

关键词：

BETA-N-ACETYLHEXOSAMIMIDASE; AUTOMATED PROCEDURE; HUMAN CEREBROSPINAL FLUID; HUMAN SERUM; MULTIPLE SCLEROSIS;

D O I：

10.1016/0009-8981(91)90079-R

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

beta-N-Acetylhexosaminidase activity and isoenzyme have been investigated in normal human cerebrospinal fluid and that of patients with multiple sclerosis. beta-N-acetylhexosaminidase activity in normal cerebrospinal fluids has been resolved into five components. The major component was in a form that eluted from DEAE cellulose at the same salt concentration as hexosaminidase As, the isoenzyme previously identified in human serum. Cerebrospinal fluid from patients exhibited a different isoenzyme profile, showing a remarkable increase in a form having a pI which was more acidic than that of As. These changes have a potential use in the diagnosis and further biochemical characterization of multiple sclerosis.

引用

页码：73 / 80

页数：8

共 14 条

[1] INTERMEDIATE FORMS OF HUMAN BETA-N-ACETYLHEXOSAMINIDASE LACK ACTIVITY TOWARDS 4-METHYLUMBELLIFERYL BETA-N-ACETYLGLUCOSAMINIDE 6-SULFATE [J].