QUANTITATIVE-ANALYSIS OF TYLOSIN BY COLUMN LIQUID-CHROMATOGRAPHY

被引：26

作者：

ROETS, E ^{[1
]}

BEIRINCKX, P ^{[1
]}

QUINTENS, I ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN, INST FARMACEUT WETENSCHAPPEN, FARMACEUT CHEM LAB, VAN EVENSTR 4, B-3000 LOUVAIN, BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 630卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80452-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A column liquid chromatographic method suitable for the quality control of tylosin A is described. The determination can be carried out on different C-8 or C18 columns, using a mobile phase containing acetonitrile, 0.2 M tetrabutylammonium hydrogensulphate, 0.2 M phosphoric acid and water. The flow-rate is 1 ml/min and detection is performed at 280 nm. The method shows good selectivity towards the major components tylosin A, B, C and D and demycinosyltylosin. Minor degradation products, mainly observed in solutions, are also separated. The compositions of several standards are compared and results for a number of commercial samples are presented.

引用

页码：159 / 166

页数：8

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