FLUORESCENCE DETECTOR CELL FOR USE IN AN INTEGRATED ELECTRICALLY DRIVEN SEPARATION SYSTEM

被引：4

作者：

DEBOKX, PK ^{[1
]}

GILLISSEN, EEA ^{[1
]}

VANDEWEIJER, P ^{[1
]}

BEKKERS, MHJ ^{[1
]}

VANBOMMEL, CHM ^{[1
]}

JANSSEN, HG ^{[1
]}

机构：

[1] EINDHOVEN UNIV TECHNOL,5600 MB EINDHOVEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 598卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)85121-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An integrated design for electrically driven separations is presented. The injector, column and detector are all located within the same cartridge, allowing for integral thermostating and short column lengths. Results on the performance of the proposed fluorescence detector are reported. Molar amounts as low as 2.10(-19) mol of fluorescein (100-pl detector cell volume) can be detected. Short column lengths permit very fast separations, as is demonstrated by the separation of four laser dyes within 35 s.

引用

收藏

页码：115 / 121

页数：7

相关论文

共 22 条

[1] SUBATTOMOLE AMINO-ACID ANALYSIS BY CAPILLARY ZONE ELECTROPHORESIS AND LASER-INDUCED FLUORESCENCE [J].

CHENG, YF ;

DOVICHI, NJ .

SCIENCE, 1988, 242 (4878) :562-564

[2]

DEBOKX PK, IN PRESS SEP SCI TEC

[3] LASER-INDUCED FLUORESCENCE DETECTION FOR CONVENTIONAL AND MICROCOLUMN LIQUID-CHROMATOGRAPHY [J].

FOLESTAD, S ;

JOHNSON, L ;

JOSEFSSON, B ;

GALLE, B .

ANALYTICAL CHEMISTRY, 1982, 54 (06) :925-929

[4] CAPILLARY ZONE ELECTROPHORESIS - QUANTITATIVE STUDY OF THE EFFECTS OF SOME DISPERSIVE PROCESSES ON THE SEPARATION EFFICIENCY [J].

FORET, F ;

DEML, M ;

BOCEK, P .

JOURNAL OF CHROMATOGRAPHY, 1988, 452 :601-613

[5] ON-COLUMN HELIUM CADMIUM LASER FLUORESCENCE DETECTOR FOR OPEN-TUBULAR CAPILLARY LIQUID-CHROMATOGRAPHY [J].

GUTHRIE, EJ ;

JORGENSON, JW ;

DLUZNESKI, PR .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1984, 22 (04) :171-176

[6] ON-COLUMN CONDUCTIVITY DETECTOR FOR CAPILLARY ZONE ELECTROPHORESIS [J].

HUANG, XH ;

PANG, TKJ ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1987, 59 (23) :2747-2749

[7] CURRENT-MONITORING METHOD FOR MEASURING THE ELECTROOSMOTIC FLOW-RATE IN CAPILLARY ZONE ELECTROPHORESIS [J].

HUANG, XH ;

GORDON, MJ ;

ZARE, RN .

ANALYTICAL CHEMISTRY, 1988, 60 (17) :1837-1838

[8] ZONE ELECTROPHORESIS IN OPEN-TUBULAR GLASS-CAPILLARIES [J].

JORGENSON, JW ;

LUKACS, KD .

ANALYTICAL CHEMISTRY, 1981, 53 (08) :1298-1302

[9] HIGH-RESOLUTION SEPARATIONS BASED ON ELECTROPHORESIS AND ELECTROOSMOSIS [J].

JORGENSON, JW ;

LUKACS, KD .

JOURNAL OF CHROMATOGRAPHY, 1981, 218 (1-3) :209-216

[10] MICROCOLUMN SEPARATIONS AND THE ANALYSIS OF SINGLE CELLS [J].

KENNEDY, RT ;

OATES, MD ;

COOPER, BR ;

NICKERSON, B ;

JORGENSON, JW .

SCIENCE, 1989, 246 (4926) :57-63