PHOTOSENSITIVITIES OF IODOPSIN AND RHODOPSINS

被引：46

作者：

OKANO, T

FUKADA, Y

SHICHIDA, Y

YOSHIZAWA, T

机构：

[1] UNIV ELECTROCOMMUN,DEPT APPL PHYS & CHEM,1-5-1 CHOFUGAOKA,CHOFU,TOKYO 182,JAPAN

[2] KYOTO UNIV,FAC SCI,DEPT BIOPHYS,KYOTO 60601,JAPAN

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1992年 / 56卷 / 06期

关键词：

D O I：

10.1111/j.1751-1097.1992.tb09722.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relative photosensitivity and the molar extinction coefficient of a highly purified iodopsin (chicken red sensitive cone visual pigment) solubilized in a mixture of 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate and phosphatidylchotine (CHAPS-PC) were measured using bovine rhodopsin solubilized in 2% digitonin as a standard and compared with those of chicken and bovine rhodopsins. The photosensitivity obtained (1.08) was close to those of rhodopsins (chicken, 1.04; bovine, 0.99) in CHAPS-PC. The molar extinction coefficient of iodopsin (47 200) was 1.15-1.17 times higher than those of rhodopsins (chicken, 40500; bovine, 41200). The oscillator strength of iodopsin (0.60) calculated from the extinction coefficient was nearly identical to that of chicken rhodopsin (0.61), suggesting that the chromophore of iodopsin is similar in configuration to rhodopsin. In contrast, the difference in quantum yield between iodopsin (0.62) and chicken rhodopsin (0.70) suggests that the chromophore-opsin interaction after absorption of a photon by the chromophore may be different.

引用

页码：995 / 1001

页数：7

共 25 条

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