INSTANT RNA ISOLATION FROM VIRUS-INFECTED TISSUE-CULTURE FLUID FOR THE POLYMERASE CHAIN-REACTION

被引：12

作者：

AFZAL, MA

MINOR, PD

机构：

[1] Division of Virology, National Institute for Biological Standards and Control, South Mimms, Potters Bar

来源：

VACCINE | 1994年 / 12卷 / 11期

关键词：

RNA ISOLATION; POLYMERASE CHAIN REACTION; METHODOLOGY;

D O I：

10.1016/0264-410X(94)90330-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This paper describes an efficient method of RNA isolation, enabling RNA templates to be obtained for reverse transcription within 20 min. The procedure avoids the use of hazardous organic chemicals and ethanol precipitation, and does not require ultracentrifugation. Results are presented for extraction of RNA from virus-infected tissue culture fluid, infected cell sheets, vaccine bulk material and lyophilized commercial vaccines.

引用

页码：976 / 977

页数：2

共 5 条

[1] USE OF SINGLE-STRAND CONFORMATION POLYMORPHISM TECHNIQUE FOR THE INITIAL CHARACTERIZATION OF VIRUS ISOLATES [J].