EFFECT OF GLPT AND GLPD MUTATIONS ON EXPRESSION OF THE PHOA GENE IN ESCHERICHIA-COLI

被引:36
作者
RAO, NN
ROBERTS, MF
TORRIANI, A
YASHPHE, J
机构
[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[2] BOSTON COLL,DEPT CHEM,CHESTNUT HILL,MA 02167
[3] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT BACTERIOL,IL-91010 JERUSALEM,ISRAEL
关键词
D O I
10.1128/JB.175.1.74-79.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In vivo P-31 nuclear magnetic resonance analysis of Escherichia coli cells showed that the intracellular concentration of P(i) remained constant in wild-type and in a glpT mutant strain whether the cells were grown on excess (2 mM) P(i) or sn-glycerol-3-phosphate as a phosphate source. The function of the phoA promoter (measured by beta-galactosidase activity in a phoA-lacZ fusion strain) was repressed when glpT+ cells were utilizing sn-glycerol-3-phosphate as the sole source of phosphate. These cells were devoid of alkaline phosphatase activity. However, the phoA promoter was fully active in a glpT mutant. These results indicated that the repression of the enzyme synthesis was not due to a variation in the level of cytoplasmic P(i) but was due to the P(i) excreted into the periplasm and/or to the medium.
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页码:74 / 79
页数:6
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