DETECTION OF SUPEROXIDE FREE-RADICALS IN RATS WITH ACUTE-PANCREATITIS

被引：19

作者：

KISHIMOTO, W

NAKAO, A

NAKANO, M

TAKAHASHI, A

INABA, H

TAKAGI, H

机构：

[1] NAGOYA UNIV, SCH MED, DEPT SURG 2, SHOWA KU, NAGOYA, AICHI 466, JAPAN

[2] HAMAMATSU UNIV SCH MED, PHOTON MED RES CTR, HAMAMATSU, SHIZUOKA 43131, JAPAN

[3] RES DEV CORP JAPAN, BIOPHOTON PROJECT, SENDAI 982, MIYAGI, JAPAN

来源：

PANCREAS | 1995年 / 11卷 / 02期

关键词：

SUPEROXIDE FREE RADICALS; ACUTE PANCREATITIS; RATS; MCLA (2-METHYL-6-[P-METHOXYPHENYL]-3,7-DIHYDROIMIDAZOL[1,2-1]PYRAZIN-3-ONE); CERULEIN; CHEMILUMINESCENCE;

D O I：

10.1097/00006676-199508000-00002

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

To study the importance of oxygen-derived free radicals in acute pancreatitis, an experimental study of in vivo detection of superoxide free radicals (O-2(-)) was performed using rats. Using a new chemiluminescence probe (2-methyl-6-[p-methoxyphenyl]-3,7-dihydro- imidazol[1,2-1]pyrazin-3-one; MCLA; a Cypridina luciferin analogue) and a highly sensitive photon counting system, O-2(-) from the pancreatic surface of rats with experimental acute pancreatitis induced by 180 mu g cerulein/kg was detected. The time course of MCLA-dependent luminescence suggested that O-2(-) production began 2-3 h after cerulein injection and then decreased gradually. Superoxide free radical production in the pancreas of rats with cerulein-induced acute pancreatitis was confirmed using MCLA-dependent chemiluminescence. This new method allows direct observation of the behavior of oxygen-derived free radicals.

引用

页码：122 / 126

页数：5

共 11 条

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