HIGH-LEVEL PRODUCTION OF RECOMBINANT INVERTASE IN HANSENULA-POLYMORPHA

被引：3

作者：

NARCIANDI, RE

RODRIGUEZ, L

RODRIGUEZ, E

DIAZ, R

DELGADO, J

HERRERA, L

机构：

[1] Center for Genetic Engineering and Biotechnology, Cubanacan, Havana

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 09期

关键词：

D O I：

10.1007/BF00127432

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The SUC 2 gene from Saccharomyces cerevisiae coding for the enzyme Invertase was cloned in Hansenula polymorpha under the control of the alcohol-oxidase (AOX) promoter of Pichia pastoris. The culture conditions for the Invertase production using a fed-batch culture were studied. More than 1,5 x 10(3) U/ml of Invertase (1 g/L) were found to be secreted to cellular periplasmic space. The scale up to 50 L of the fermentative process was done.

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页码：949 / 952

页数：4

相关论文

共 11 条

[1] HIGH-LEVEL EXPRESSION OF TETANUS TOXIN FRAGMENT-C IN PICHIA-PASTORIS STRAINS CONTAINING MULTIPLE TANDEM INTEGRATIONS OF THE GENE [J].

CLARE, JJ ;

RAYMENT, FB ;

BALLANTINE, SP ;

SREEKRISHNA, K ;

ROMANOS, MA .

BIO-TECHNOLOGY, 1991, 9 (05) :455-460

[2] RECENT ADVANCES IN THE EXPRESSION OF FOREIGN GENES IN PICHIA-PASTORIS [J].

CREGG, JM ;

VEDVICK, TS ;

RASCHKE, WC .

BIO-TECHNOLOGY, 1993, 11 (08) :905-910

[3] HIGH-LEVEL EXPRESSION AND EFFICIENT ASSEMBLY OF HEPATITIS-B SURFACE-ANTIGEN IN THE METHYLOTROPHIC YEAST, PICHIA-PASTORIS [J].

CREGG, JM ;

TSCHOPP, JF ;

STILLMAN, C ;

SIEGEL, R ;

AKONG, M ;

CRAIG, WS ;

BUCKHOLZ, RG ;

MADDEN, KR ;

KELLARIS, PA ;

DAVIS, GR ;

SMILEY, BL ;

CRUZE, J ;

TORREGROSSA, R ;

VELICELEBI, G ;

THILL, GP .

BIO-TECHNOLOGY, 1987, 5 (05) :479-485

[4] PRODUCTION AND APPLICATION OF METHYLOTROPHIC YEAST PICHIA-PASTORIS [J].

DUFF, SJB ;

MURRAY, WD .

BIOTECHNOLOGY AND BIOENGINEERING, 1988, 31 (01) :44-49

[5] PRODUCTION OF CATALASE-FREE ALCOHOL OXIDASE BY HANSENULA-POLYMORPHA [J].

GIUSEPPIN, MLF ;

VANEIJK, HMJ ;

VERDUYN, C ;

BANTE, I ;

VANDIJKEN, JP .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1988, 28 (01) :14-19

[6]

Montesino R., 1992, Biotecnologia Aplicada, V9, P22

[7] TRANSFORMATION OF THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA BY AUTONOMOUS REPLICATION AND INTEGRATION VECTORS [J].

ROGGENKAMP, R ;

HANSEN, H ;

ECKART, M ;

JANOWICZ, Z ;

HOLLENBERG, CP .

MOLECULAR & GENERAL GENETICS, 1986, 202 (02) :302-308

[8] FOREIGN GENE-EXPRESSION IN YEAST - A REVIEW [J].

ROMANOS, MA ;

SCORER, CA ;

CLARE, JJ .

YEAST, 1992, 8 (06) :423-488

[9] ISOLATION OF L-METHIONINE-ENRICHED MUTANT OF A METHYLOTROPHIC YEAST, CANDIDA-BOIDINII NO 2201 [J].

TANI, Y ;

LIM, WJ ;

YANG, HC .

JOURNAL OF FERMENTATION TECHNOLOGY, 1988, 66 (02) :153-158

[10] NUCLEOTIDE-SEQUENCE OF THE YEAST SUC2 GENE FOR INVERTASE [J].

TAUSSIG, R ;

CARLSON, M .

NUCLEIC ACIDS RESEARCH, 1983, 11 (06) :1943-1954