MULTIPLE CLONING SITES CARRYING LOXP AND FRT RECOGNITION SITES FOR THE CRE AND FLP SITE-SPECIFIC RECOMBINASES

被引:17
作者
SNAITH, MR
MURRAY, JAH
BOULTER, CA
机构
[1] UNIV CAMBRIDGE, INST BIOTECHNOL, CAMBRIDGE CB2 1QT, ENGLAND
[2] UNIV CAMBRIDGE, DEPT GENET, CAMBRIDGE CB2 3EH, ENGLAND
基金
英国医学研究理事会;
关键词
RECOMBINANT DNA TOOLS; SITE-SPECIFIC RECOMBINATION; BACTERIOPHAGE-P1; 2-MU CIRCLE PLASMID;
D O I
10.1016/0378-1119(95)00579-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Plasmids were constructed carrying the loxP and FRT recognition sites for the Cre and Flp site-specific recombinases, respectively, within multiple cloning sites. Vectors carrying single and tandemly repeated targets are available with various flanking restriction enzyme sites. In addition, a series of plasmids carrying both loxP and FRT sites is available. These vectors facilitate construction of target molecules for these site-specific recombinases which are becoming increasingly important tools for the in vivo manipulation of DNA.
引用
收藏
页码:173 / 174
页数:2
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