DETERMINATION OF PROTEINASE 3-ALPHA(1)-ANTITRYPSIN COMPLEXES IN INFLAMMATORY FLUIDS

被引：30

作者：

DOLMAN, KM

VANDEWIEL, BA

KAM, CM

ABBINK, JJ

HACK, CE

SONNENBERG, A

POWERS, JC

VONDEMBORNE, AEGK

GOLDSCHMEDING, R ^{[1
]}

机构：

[1] NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,PUBLICAT SECRETARIAT,POB 9406,1006 AK AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,EXPTL & CLIN IMMUNOL LAB,AMSTERDAM,NETHERLANDS

[3] GEORGIA INST TECHNOL,SCH CHEM & BIOCHEM,ATLANTA,GA 30332

[4] UNIV AMSTERDAM HOSP,ACAD MED CTR,DEPT HAEMATOL,AMSTERDAM,NETHERLANDS

来源：

FEBS LETTERS | 1992年 / 314卷 / 02期

关键词：

PROTEINASE; 3; ALPHA(1)-ANTITRYPSIN; INFLAMMATORY FLUID;

D O I：

10.1016/0014-5793(92)80955-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is alpha1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-alpha1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-alpha1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-alpha1AT complexes. Thus, in vivo alpha1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

引用

页码：117 / 121

页数：5

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