CHARACTERIZATION OF A LINEAR EXTRACHROMOSOMAL DNA ELEMENT (PBL1) ISOLATED AFTER INTERSPECIFIC MATING BETWEEN STREPTOMYCES-BAMBERGIENSIS AND STREPTOMYCES-LIVIDANS

被引：17

作者：

ZOTCHEV, SB ^{[1
]}

SOLDATOVA, LI ^{[1
]}

OREKHOV, AV ^{[1
]}

SCHREMPF, H ^{[1
]}

机构：

[1] MOSCOW GENET & SELECT IND MICROORGANISMS INST, MOSCOW, RUSSIA

来源：

RESEARCH IN MICROBIOLOGY | 1992年 / 143卷 / 09期

关键词：

DNA; PLASMID; MATING; STREPTOMYCES-BAMBERGIENSIS; STREPTOMYCES-LIVIDANS; TRANSFER; LINEAR; PHYSICAL MAP;

D O I：

10.1016/0923-2508(92)90071-U

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Streptomyces bambergiensis S712 harbours a giant linear plasmid PSB1 of 640 kb. After mating with the plasmidless S. lividans strain TK64, conjugants carrying a smaller extrachromosomal DNA element, pBL1, were identified. pBL1 is a 43-kb linear DNA molecule bound to a protein which protects it from attack by both 3'- and 5'-exonucleases. The absence of this protein drastically reduces the transforming efficiency of pBL1. pBL1 shares homology with linear plasmids and chromosomal DNA from S. bambergiensis strains.

引用

页码：839 / 845

页数：7

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