CORRELATION BETWEEN INSERTION MUTANT ACTIVITIES AND AMINO-ACID-SEQUENCE IDENTITIES OF THE TAQI AND TTHHB8 RESTRICTION ENDONUCLEASES

被引：9

作者：

BARANY, F

ZEBALA, J

机构：

[1] Department of Microbiology, Hearst Microbiology Research Center, Cornell University Medical College, New York

来源：

GENE | 1992年 / 112卷 / 01期

关键词：

ISOSCHIZOMERS; GEMINI PROTEINS; PROTEIN DNA INTERACTIONS; RECOMBINANT DNA; 2-CODON INSERTION MUTAGENESIS;

D O I：

10.1016/0378-1119(92)90297-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A two-codon insertion mutagenesis method has been generalized. Over two dozen insertion mutants throughout the gene encoding TaqI restriction endonuclease were constructed and activity was characterized. All mutants with activity either cleaved or nicked the canonical T down CGA recognition sequence. Some insertion mutants created duplication of gene regions, termed Gemini proteins, which still retained activity. The correlation between mutants with poor activity and the regions of shared amino acid identity between the isoschizomeric TaqI and TthHB8I suggests these regions are involved in DNA recognition and/or catalysis.

引用

页码：13 / 20

页数：8

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