DETERMINATION OF LIPASE SPECIFICITIES THROUGH THE USE OF CHIRAL TRIGLYCERIDES AND THEIR RACEMICS

被引：27

作者：

VILLENEUVE, P ^{[1
]}

PINA, M ^{[1
]}

MONTET, D ^{[1
]}

GRAILLE, J ^{[1
]}

机构：

[1] CIRAD,CP,LIPOTECH LAB,F-34032 MONTPELLIER,FRANCE

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1995年 / 76卷 / 01期

关键词：

MODEL TRIGLYCERIDES; STEREOSPECIFICITY; TYPOSELECTIVITY; LIPASE; RABBIT GASTRIC LIPASE; CANDIDA ANTARTICA; PSEUDOMONAS AERUGINOSA;

D O I：

10.1016/0009-3084(95)02421-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The previously published synthesis of defined chiral triglycerides has been extended to the synthesis of their racemics. The combined use of the chiral-racemic pair of a triglyceride can now be used to accurately define the effective specificity of a large number of lipases. As an example, we confirm unambiguously in this paper, the sn3 stereospecificity for the rabbit gastric lipase, the short chain typoselectivity for the Candida antartica lipase and the sn1 stereospecificity for the Pseudomonas aeruginosa lipase.

引用

页码：109 / 113

页数：5

共 15 条

[1] SPECIFICATION OF MOLECULAR CHIRALITY [J].