FACTORS AFFECTING THE IN-VITRO RAPID GERMINATION OF TAXUS EMBRYOS AND THE EVALUATION OF TAXOL CONTENT IN THE PLANTLETS

被引：41

作者：

ZHIRI, A

JAZIRI, M

HOMES, J

VANHAELEN, M

SHIMOMURA, K

机构：

[1] FREE UNIV BRUSSELS,PHARMACOGNOSY & BROMATOL LAB,B-1050 BRUSSELS,BELGIUM

[2] NATL INST HYG SCI,TSUKUBA MED PLANT RES STN,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1994年 / 39卷 / 03期

关键词：

EMBRYO CULTURE; TAXACEAE; YEW;

D O I：

10.1007/BF00035979

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and efficient in vitro method for breaking the dormancy of Taxus baccata L. cv. Stricta seeds was investigated. The highest rate of germination (100%) of embryos isolated from seeds, which had been washed with running tap water for 7 days, was obtained after 7 days of culture on modified Murashige and Skoog or Helier media. The taxol equivalent content in 2-month-old Taxus plantlets was investigated using anti-taxol polyclonal antibodies. The results showed that the taxol equivalent content varied, depending on Taxus species and on the individuals in the same taxon.

引用

页码：261 / 263

页数：3

共 14 条

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