INCIDENCE OF LYSOGENY IN WILD LACTOCOCCAL STRAINS

被引:12
作者
CUESTA, P [1 ]
SUAREZ, JE [1 ]
RODRIGUEZ, A [1 ]
机构
[1] CSIC,INST PORD LACTEOS ASTURIAS,E-33300 VILLAVICIOSA,SPAIN
关键词
LYSOGENY; LACTOCOCCI; FARMHOUSE CHEESE;
D O I
10.3168/jds.S0022-0302(95)76715-7
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The incidence of lysogeny among 172 lactococcal strains, isolated from natural dairy fermentations and selected by their high capacity to coagulate milk and to produce lactic acid, was studied. Lysis of 92 strains (53% of the strains tested) was observed after using mitomycin C for induction of cultures. Fifty-one of these 92 strains released phages that were able to propagate on indicator strains. Twenty-eight (16%) of the 172 strains tested acted as host for at least one phage, although some supported the development of more than one phage isolate. Spontaneous induction was insignificant in >50% of strains inducible by mitomycin C, but titers after mitomycin C induction varied between 10(1) and 10(6) pfu/ml. No plaque formation was observed at 37 degrees C. The bacteriophages belonged to the family Siphoviridae, as revealed by electron microscopy. Head and tail dimensions ranged widely. Some temperate phages that were unable to form plaques of lysis appeared to be defective viral particles.
引用
收藏
页码:998 / 1003
页数:6
相关论文
共 24 条
[1]  
Ackermann H.W., Cantor C.D., Jarvis A.W., Mayo J.A., New species definitions in phages of Gram-positive cocci, Intervirology, 22, (1984)
[2]  
Davidson B.E., Powell I.B., Hillier A.J., Temperate bacteriophages and lysogeny in lactic acid bacteria, Fed. Eur. Microbiol. Soc. Microbiol. Rev., 87, (1990)
[3]  
Davies F.L., Gasson M.J., Reviews of the progress of dairy science: genetics of lactic acid bacteria, J. Dairy Res., 48, (1981)
[4]  
Gasson M.J., Davies F.L., Prophagecured derivatives of Streptococcuslactis and Strep tococcus cremoris, Appl. Environ. Microbiol., 40, (1980)
[5]  
Heap H.A., Limsowtin G.K.Y., Lawrence R.C., Contribution of Streptococcuslactis strains in raw milk to phage infection in commercial cheese factories, N. Z. I. Dairy Sci. Technol., 13, (1978)
[6]  
Huggins A.R., Sandine W.E., Incidence and properties of temperate bacteriophages induced from lactic streptococci, Appl. Environ. Microbiol., 33, (1977)
[7]  
Milk and Milk Products. Methods of Sampling, (1985)
[8]  
Jarvis A.W., Bacteriophages of lactic acid bacteria, Journal of Dairy Science, 72, (1989)
[9]  
Jarvis A.W., Fitzgerald G.F., Mata M., Mercenier A., Neve H., Powell I.B., Ronda C., Saxelin M., Teuber M., Species and type phages of lactococcal bacteriophages, Intervirology, 32, (1991)
[10]  
Kozak W., Rajchert-Trzpil M., Zajdel J., Dobrzanski W.T., Lysogeny in lactic acid streptococci producing and not producing nisin, Appl. Microbiol., 25, (1973)