ANTIPEPTIDE ANTIBODIES AGAINST THE PNR-2 ESTROGEN-REGULATED PROTEIN OF HUMAN BREAST-CANCER CELLS AND DETECTION OF PNR-2 EXPRESSION IN NORMAL-TISSUES BY IMMUNOHISTOCHEMISTRY

被引：85

作者：

PIGGOTT, NH ^{[1
]}

HENRY, JA ^{[1
]}

MAY, FEB ^{[1
]}

WESTLEY, BR ^{[1
]}

机构：

[1] ROYAL VICTORIA INFIRM,DEPT PATHOL,NEWCASTLE TYNE NE1 4LP,TYNE & WEAR,ENGLAND

来源：

JOURNAL OF PATHOLOGY | 1991年 / 163卷 / 02期

关键词：

PNR-2/PS2; ESTROGEN REGULATION; IMMUNOHISTOCHEMISTRY; NORMAL BREAST; SMALL INTESTINE; STOMACH;

D O I：

10.1002/path.1711630204

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Five peptides, corresponding to regions of the predicted protein sequence of the oestrogen-regulated pNR-2 protein which is expressed in oestrogen-responsive human breast cancer cells, were synthesized. Two peptides were immunogenic in rabbits and antisera against one peptide reacted with the pNR-2 protein in sections of formalin-fixed, paraffin-embedded breast tumour. There was a significant correlation between the extent of pNR-2 protein expression detected by immunohistochemistry and pNR-2 mRNA levels determined by hybridization with a cDNA probe in a series of primary breast tumours. pNR-2 expression was assessed immunohistochemically in a panel of normal tissues. Expression was detected in normal breast, small intestine, and stomach (body and antrum).

引用

页码：95 / 104

页数：10

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