ENZYME IMMUNOMETRIC ASSAY FOR L-THYROXINE USING DIRECT ULTRAVIOLET-IRRADIATION

被引：12

作者：

ETIENNE, E

CREMINON, C

LAMOURETTE, P

GRASSI, J

PRADELLES, P

机构：

[1] CEA, Service de Pharmacologic et d'lmmunologie, Département de Recherche en Imagerie, Pharmacologic et Physiologie, CE Saclay

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 225卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1104

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new enzyme immunometric assay for L-thyroxine using uv irradiation as a cross-linking procedure is described. L-Thyroxine in plasma samples is immunocaptured by a monoclonal anti-L-thyroxine antibody coated on 96-well microtiter plates, After uv irradiation and methanol treatment, the covalently linked L-thyroxine is measured using the same monoclonal anti-L-thyroxine antibody labeled with acetylcholinesterase. A minimal detectable concentration of 4.8 nmol/liter was observed with a coefficient of variation less than 16% in the 20-320 nmol/liter. Specificity of the assay was very satisfying and a good correlation (r = 0.959) was noted for 33 human plasma samples between this assay and a commercial competitive radioimmunoassay. (C) 1995 Academic Press, Inc.

引用

页码：34 / 38

页数：5

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