STRUCTURE OF THE PUTATIVE O-10 ANTIGEN FROM ACINETOBACTER-BAUMANNII

被引：26

作者：

HASELEY, SR ^{[1
]}

WILKINSON, SG ^{[1
]}

机构：

[1] UNIV HULL,SCH CHEM,KINGSTON HULL HU6 7RX,N HUMBERSIDE,ENGLAND

来源：

CARBOHYDRATE RESEARCH | 1994年 / 264卷 / 01期

关键词：

ACINETOBACTER BAUMANNII; LIPOPOLYSACCHARIDE; O ANTIGEN;

D O I：

10.1016/0008-6215(94)00179-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose was obtained from an aqueous phenol extract of isolated cell walls from the reference strain for Acinetobacter baumannii serogroup 010. By means of NMR studies and chemical degradations, the repeating unit of the polymer (the putative 010 antigen) was identified as a branched pentasaccharide of the structure shown. [GRAPHICS] at O-2 and O-4 with alpha-D-Gal p and beta-D-Gal p, respectively. The fractions differed in that one contained much more uronic acid than the other, with corresponding predominance of side chains containing alpha-D-Glc p A-(1 --> 3)-D-Glc p, (alpha-D-Gal p-(1 -->, 2)-D-Glc p, and beta-D-Gal f units.

引用

页码：73 / 81

页数：9

共 32 条

[1] STRUCTURAL STUDIES OF THE O-ANTIGEN OF THE LIPOPOLYSACCHARIDE FROM AN AVIRULENT STRAIN (M4S) OF PSEUDOMONAS-SOLANACEARUM [J].

AKIYAMA, Y ;

EDA, S ;

KATO, K .

CARBOHYDRATE RESEARCH, 1984, 133 (02) :289-296

[2]

[Anonymous], METHODS ENZYMOL

[3] SEMIMICRO DETERMINATION OF SUGAR CONFIGURATION BY MEASUREMENT OF CIRCULAR-DICHROISM OF ALDITOL ACETATES [J].

BEBAULT, GM ;

BERRY, JM ;

CHOY, YM ;

DUTTON, GGS ;

FUNNELL, N ;

HAYWARD, LD ;

STEPHEN, AM .

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE, 1973, 51 (02) :324-326

[4] STUDY OF C-13H COUPLING-CONSTANTS IN HEXOPYRANOSES [J].

BOCK, K ;

PEDERSEN, C .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1974, (03) :293-299

[5] OUTER-MEMBRANE VESICULATION OF ACINETOBACTER CALCOACETICUS [J].

BORNELEIT, P ;

BINDER, H ;

KLEBER, HP .

ACTA BIOTECHNOLOGICA, 1990, 10 (02) :117-123

[6] PREPARATION OF R-TYPE LIPOPOLYSACCHARIDES OF ACINETOBACTER-CALCOACETICUS BY EDTA-SALT EXTRACTION [J].

BORNELEIT, P ;

BLECHSCHMIDT, B ;

BLASIG, R ;

FRANKE, P ;

GUNTHER, P ;

KLEBER, HP .

CURRENT MICROBIOLOGY, 1989, 19 (02) :77-81

[7]

BROWN DH, 1974, MEHTODS ENZYMATI ANA, V3, P1251

[8] STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF XANTHOMONAS-CAMPESTRIS NCPPB-45 LIPOPOLYSACCHARIDE [J].

BUKHAROV, AV ;

SKVORTSOV, IM ;

IGNATOV, VV ;

SHASHKOV, AS ;

KNIREL, YA ;

DABROWSKI, J .

CARBOHYDRATE RESEARCH, 1993, 241 :309-316

[9]

DIFABIO JL, 1989, BIOCHEM CELL BIOL, V67, P78

[10]

DIFABIO JL, 1988, BIOCHEM CELL BIOL, V66, P107

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