CHARACTERIZATION OF PPVUL, THE AUTONOMOUS PLASMID FROM PROTEUS-VULGARIS THAT CARRIES THE GENES OF THE PVUII RESTRICTION-MODIFICATION SYSTEM

被引：20

作者：

CALVINKOONS, MD ^{[1
]}

BLUMENTHAL, RM ^{[1
]}

机构：

[1] MED COLL OHIO,DEPT MICROBIOL,TOLEDO,OH 43699

来源：

GENE | 1995年 / 157卷 / 1-2期

关键词：

PLASMID REPLICATION; INCOMPATIBILITY; RECOMBINATION; RNA I; ROM; CER;

D O I：

10.1016/0378-1119(94)00618-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmid pPvu1 from Proteus vulgaris carries the genes of the PvuII restriction-modification system [Blumenthal et al., J. Bacteriol. 164 (1985) 501-509]. This report focuses on physical and functional features of the 4.84-kb plasmid, which shows a composite genetic architecture. Plasmid pPvu1 has a replication origin and an incompatibility locus that each function in Escherichia coli, and an apparent cer recombination site. The replication origin includes a possible RNAI gene, and the incompatibility locus closely resembles a rom gene. These loci show substantial sequence similarity to corresponding loci from the E. coli plasmids P15A, ColEI and pSC101, and closely flank the PvuII genes. The close association between a recombinational locus and the PvuII genes has implications for their mobility.

引用

页码：73 / 79

页数：7

共 39 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE OF THE PVU-II RESTRICTION ENZYME GENE FROM PROTEUS-VULGARIS [J].