Effect of Dentin Biomodification Using Naturally Derived Collagen Cross-Linkers: One-Year Bond Strength Study

Purpose. This study investigated the long-term resin-dentin bond strength of dentin biomodified by proanthocyanidin-rich (PA) agents. Materials and Methods. Forty molars had their coronal dentin exposed, etched, and treated for 10 minutes with 6.5% grape seed extract (GSE), 6.5% cocoa seed extract ethanol-water (CSE-ET), 6.5% cocoa seed extract acetone-water (CSE-AC), and distilled water (CO). Samples were restored either with One-Step Plus (OS) or Adper Single-Bond Plus (SB). Bond strength test was performed immediately or after 3, 6, and 12 months. Results. Higher mu TBS were observed for GSE immediately (SB62.9MPa; OS-51.9MPa) when compared to CSE-ET (SB-56.95MPa; OS-60.28MPa), CSE-AC (SB-49.97MPa; OS-54.44MPa), and CO (SB-52.0MPa; OS-44.0MPa) (P < 0.05). CSE outcomes were adhesive system and solvent dependant. After 12 months storage SB results showed no difference among treatment types (GSE-57.15MPa; CSE/ET-54.04MPa; CSE/AC-48.22MPa; CO51.68MPa; P = 0.347), while OS results where treatment dependent (GSE-42.62MPa; CSE/ET-44.06MPa; CSE/AC-41.30MPa; CO-36.85MPa; P = 0.036). Conclusions. GSE and CSE-ET agents provided enhanced immediate adhesion and stabilization to demineralized dentin after long-term storage, depending on adhesive system.