INFLUENCE OF GRAPHITE POWDER, ADDITIVES AND ENZYME IMMOBILIZATION PROCEDURES ON A MEDIATORLESS HRP-MODIFIED CARBON-PASTE ELECTRODE FOR AMPEROMETRIC FLOW-INJECTION DETECTION OF H2O2

被引：58

作者：

POPESCU, IC ^{[1
]}

ZETTERBERG, G ^{[1
]}

GORTON, L ^{[1
]}

机构：

[1] LUND UNIV, DEPT ANALYT CHEM, S-22100 LUND, SWEDEN

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 05期

关键词：

MODIFIED CARBON PASTE ELECTRODES; ADDITIVES; GRAPHITE POWDERS; PEROXIDASE; HYDROGEN PEROXIDE; AMPEROMETRIC BIOSENSORS;

D O I：

10.1016/0956-5663(95)96891-2

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The behaviour of horseradish peroxidase modified carbon paste electrodes (HRP-CPEs) for mediatorless amperometric flow-injection (FI) detection of hydrogen peroxide is described. The influence of various commercially available graphite powders and additives was studied, using adsorption and covalent binding (at different pHs) as enzyme immobilization techniques. The HRP-CPE was used as the working electrode in an FI system. An optimal applied potential for electrocatalytic reduction of H2O2 was found at -50 mV vs. Ag/AgCl (0.1 M KCl). The highest sensitivity (approximate to 78 mu A cm(-2) mM(-1)) obtained in the FI made was observed for electrodes made of graphite powder having the highest surface area, with HRP immobilized by adsorption (at pH 8) in the presence of lactitol as enzyme stabilizer. The lowest detection limit (approximate to 0.1 mu M) was noted for HRP-CPE incorporating the graphite powder having the lowest background current.

引用

页码：443 / 461

页数：19

共 69 条

[1] PREPARATION AND CHARACTERIZATION OF A FRAGILE ENZYME IMMOBILIZED CARBON PASTE ELECTRODE [J].