INSITU TRANSCRIPTION - SPECIFIC SYNTHESIS OF COMPLEMENTARY-DNA IN FIXED TISSUE-SECTIONS

被引：86

作者：

TECOTT, LH ^{[1
]}

BARCHAS, JD ^{[1
]}

EBERWINE, JH ^{[1
]}

机构：

[1] STANFORD UNIV, MED CTR,SCH MED,DEPT PSYCHIAT & BEHAV SCI, NANCY PRITZKER LAB BEHAV NEUROCHEM, STANFORD, CA 94305 USA

来源：

SCIENCE | 1988年 / 240卷 / 4859期

关键词：

D O I：

10.1126/science.2454508

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A technique, in situ transcription, is described, in which reverse transcription of mRNAs is achieved within fixed tissue sections. An oligonucleotide complementary to proopiomelanocortin (POMC) mRNA was used as a primer for the specific synthesis of radiolabeled POMC cDNA in fixed sections of rat pituitary, thus permitting the rapid anatomical localization of POMC mRNA by autoradiography. Intermediate lobe signal intensities were sensitive to dopaminergic drugs, demonstrating that the method can be used for studies of mRNA regulation. The transcripts may also be eluted from tissue sections for a variety of uses, including the identification and cloning of autoradiographically localized cDNAs from small amounts of tissue.

引用

页码：1661 / 1664

页数：4

共 32 条

[1] PRIMER REQUIREMENT AND TEMPLATE SPECIFICITY OF DNA POLYMERASE OF RNA TUMOR VIRUSES - (AVIAN MYELOBLASTOSIS VIRUS/MOUSE LEUKEMIA VIRUS/E-COLI DNA POLYMERASE/HOMOPOLYNUCLEOTIDES/OLIGONUCLEOTIDES) [J].