IMMUNOLOGICAL AND HPLC DETECTION OF AFLATOXIN ADDUCTS IN HUMAN TISSUES AFTER AN ACUTE-POISONING INCIDENT IN SE ASIA

被引：38

作者：

HARRISON, JC

GARNER, RC

机构：

[1] Cancer Research Unit, Department of Biology, University of York, Heslington, York Y01 5DD, N. Yorkshire

来源：

CARCINOGENESIS | 1991年 / 12卷 / 04期

关键词：

D O I：

10.1093/carcin/12.4.741

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Acid hydrolysed, purified DNA, extracted from formalin fixed human tissues from persons acutely exposed to aflatoxins during a poisoning incident, was found to inhibit antibody binding in a competitive aflatoxin inhibition ELISA both before and after immunoaffinity column purification. HPLC analysis of acid hydrolysates of the DNA revealed a peak with a retention time 3 min earlier than 8,9-dihydro-2-(N7-guanyl)-9-hydroxy AFB1 synthesized either by peracid activation or direct reaction of the 8,9-oxide with DNA. The major peak seen when DNA was extracted from formalin fixed tissues from rats treated with aflatoxin B1 was identical to that seen in the formalin fixed human tissues. Adduct levels ranged from 0 to 170 x 10(6) nucleotides depending on tissue type and individual examined.

引用

页码：741 / 743

页数：3

共 24 条

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