HYPOTHERMIA TRIGGERS IRON-DEPENDENT LIPOPEROXIDATIVE DAMAGE IN THE ISOLATED RAT-HEART

被引：36

作者：

MAGNI, F ^{[1
]}

PANDURI, G ^{[1
]}

PAOLOCCI, N ^{[1
]}

机构：

[1] UNIV PERUGIA,CATTEDRA FISIOL UMANA,IST PATOL GEN,PERUGIA,ITALY

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1994年 / 16卷 / 04期

关键词：

RAT MYOCARDIUM; MYOCARDIUM; COLD INJURY; LIPID PEROXIDATION; IRON CHELATORS; FREE RADICALS;

D O I：

10.1016/0891-5849(94)90124-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extent and time course of recovery on return to normothermia were studied in isolated rat hearts, perfused with oxygen-saturated Tyrode's solution at 5-7 degrees C. After a 1-h hypothermia period, complete recovery was obtained on rewarming; after 3 h hypothermia irreversible deterioration of electrical and mechanical activities resulted. The level of lipid peroxidation, evaluated by the thiobarbituric acid (TBA) reaction, showed a dramatic transient increase on return to normothermia, accompanied by a decrease in the levels of reduced glutathione (GSH). Perfusion with iron chelator-containing saline completely prevented both the deterioration and the peak of lipid peroxidation. These results show that lipid peroxidation is responsible for the cold injury. It is proposed that lipid peroxidation is produced as the result of a cold-induced oxidative stress.

引用

页码：465 / 476

页数：12

共 57 条

[41]

ROMASCHIN AD, 1988, OXYGEN RADICALS PATH, P177

[42] ESTIMATION OF TOTAL PROTEIN-BOUND AND NONPROTEIN SULFHYDRYL GROUPS IN TISSUE WITH ELLMANS REAGENT [J].

SEDLAK, J ;

LINDSAY, RH .

ANALYTICAL BIOCHEMISTRY, 1968, 25 (1-3) :192-&

[43]

SHLAFER M, 1982, J THORAC CARDIOV SUR, V83, P830

[44]

Sies H, 1985, OXIDATIVE STRESS, P41

[45]

SINGAL PK, 1988, OXYGEN RADICALS PATH

[46]

SLATER TF, 1984, BIOCHEM J, V222, P1

[47]

SLATER TF, 1985, NATO ASI SERIES A, V146, P1

[48]

SMITH MT, 1985, NATO ASI SERIES A, V146, P157

[49]

STEWART JR, 1983, J THORAC CARDIOV SUR, V86, P262

[50]

SVINGEN BA, 1979, J BIOL CHEM, V254, P5892

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