HIGH-THROUGHPUT RAPID YEAST DNA EXTRACTION - APPLICATION TO YEAST ARTIFICIAL CHROMOSOMES AS POLYMERASE CHAIN-REACTION TEMPLATES

被引：8

作者：

BLANCHARD, MM ^{[1
]}

NOWOTNY, V ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,CTR GENET MED,ST LOUIS,MO 63110

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1994年 / 11卷 / 01期

关键词：

D O I：

10.1016/1050-3862(94)90003-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automation-assisted method enables DNA extraction of over a thousand yeast colonies in a day by one person. Yeast DNA is extracted essentially quantitatively in three steps: (a) cells are converted into spheroplasts with yeast lytic enzyme, (b) cells are lysed with proteinase K, and (c) DNA is collected by ethanol precipitation. The DNA stored at 4 degrees C is active in polymerase chain reaction experiments for more than a year.

引用

页码：7 / 11

页数：5

共 12 条

[1] CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].

ALBERTSEN, HM ;

ABDERRAHIM, H ;

CANN, HM ;

DAUSSET, J ;

LEPASLIER, D ;

COHEN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260

[2]

Blanchard M M, 1993, PCR Methods Appl, V2, P234

[3] CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].

BURKE, DT ;

CARLE, GF ;

OLSON, MV .

SCIENCE, 1987, 236 (4803) :806-812

[4] MAPPING HUMAN-CHROMOSOMES BY WALKING WITH SEQUENCE-TAGGED SITES FROM END FRAGMENTS OF YEAST ARTIFICIAL CHROMOSOME INSERTS [J].

KERE, J ;

NAGARAJA, R ;

MUMM, S ;

CICCODICOLA, A ;

DURSO, M ;

SCHLESSINGER, D .

GENOMICS, 1992, 14 (02) :241-248

[5] SIMPLE, RAPID, AND SENSITIVE DNA ASSAY PROCEDURE [J].

LABARCA, C ;

PAIGEN, K .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :344-352

[6]

LEE J, 1982, GENOMICS, V12, P526

[7] AN AUTOMATED-METHOD FOR DNA PREPARATION FROM THOUSANDS OF YAC CLONES [J].

MACMURRAY, AJ ;

WEAVER, A ;

SHIN, HS ;

LANDER, ES .

NUCLEIC ACIDS RESEARCH, 1991, 19 (02) :385-390

[8]

MANIATIS T, 1982, MOL CLONING

[9]

NOWOTNY V, 1991, P INT S LABORATORY A, P333

[10]

SAIKI RK, 1988, SCIENCE, V239, P87

← 1 2 →