VIP INHIBITS N-TYPE CA2+ CHANNELS OF SYMPATHETIC NEURONS VIA A PERTUSSIS TOXIN-INSENSITIVE BUT CHOLERA TOXIN-SENSITIVE PATHWAY

被引：92

作者：

ZHU, Y

IKEDA, SR

机构：

[1] Department of Pharmacology, Toxicology Medical College of Georgia, Augusta

来源：

NEURON | 1994年 / 13卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0896-6273(94)90033-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The best characterized Ca2+ channel modulation in mam malian sympathetic neurons is an inhibition of N-type channels via a pertussis toxin (PTX)-sensitive heterotrimeric G protein. Here, we show that vasoactive intestinal polypeptide (VIP), an abundant neuropeptide in the PNS and CNS, inhibited N-type Ca2+ channels in rat sympathetic neurons in a voltage-dependent, membrane-delimited manner. The effect of VIP was insensitive to PTX but was attenuated by cholera toxin or anti-G(s alpha) antibodies. VIP-mediated inhibition was independent of cAMP-dependent protein kinase A (PKA). The results provide evidence for a new signal transduction pathway in which N-type Ca2+ channel modulation requires activation of G(s alpha) but is independent of PKA-mediated phosphorylation.

引用

页码：657 / 669

页数：13

共 61 条

[1] VASOACTIVE INTESTINAL POLYPEPTIDE INCREASES INOSITOL PHOSPHOLIPID BREAKDOWN IN THE RAT SUPERIOR CERVICAL-GANGLION [J].