IMMUNOSUPPRESSION BY PULMONARY SURFACTANT - MECHANISMS OF ACTION

被引:42
作者
WILSHER, ML [1 ]
PARKER, DJ [1 ]
HASLAM, PL [1 ]
机构
[1] NATL HEART & LUNG INST,DEPT CARDIOTHORAC SURG,CELL BIOL UNIT,LONDON SW3 6LY,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1136/thx.45.1.3
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Pulmonary surfactant has been shown by this group to suppress peripheral blood lymphocyte responses to mitogens and alloantigens in a dose dependent manner, though the mechanism of action of the suppressive effect is not clearly understood. To try to clarify this, attempts were made to reverse the effects of preincubation with surfactant, obtained by bronchoalveolar lavage from pigs, on lymphocytes and accessory monocytes obtained from the blood of normal volunteers, by washing and incubating the cells in medium alone for various periods up to 24 hours. Immunosuppression, measured as the reduction in thymidine incorporation in response to the mitogen phytohaemagglutinin, could not be reversed by these methods. The addition of indomethacin (up to 100 μg/ml for 72 hours) also had no effect, indicating that the immunosuppression was not related to synthesis of prostaglandins. Incubation with surfactant for as little as two hours before addition of mitogen suppressed in vitro lymphoproliferative responses by half, but surfactant added two hours after mitogen had no observed effect. Preincubation of purified lymphocytes in surfactant, before they were cultured with accessory monocytes and mitogen, caused significant suppression of response, but preincubation of purified monocytes had no suppressive effect. There was no change in the intensity of HLA-DR expression on monocytes. These results support the hypothesis that surfactant exerts its effects on the resting uncommitted lymphocyte rather than on antigen presenting monocytes.
引用
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页码:3 / 8
页数:6
相关论文
共 26 条
[1]  
ANSFIELD MJ, 1979, J IMMUNOL, V122, P1062
[2]  
CURTISS LK, 1977, J IMMUNOL, V118, P1966
[3]  
CURTISS LK, 1977, J IMMUNOL, V199, P2129
[4]  
DAVIS L, 1986, J IMMUNOL, V136, P3588
[5]  
DEMENKOFF JH, 1980, J IMMUNOL, V124, P1365
[6]   HUMAN ALVEOLAR MACROPHAGE SUPPORT OF LYMPHOCYTE-RESPONSES TO MITOGENS AND ANTIGENS - ANALYSIS AND COMPARISON WITH AUTOLOGOUS PERIPHERAL-BLOOD-DERIVED MONOCYTES AND MACROPHAGES [J].
ETTENSOHN, DB ;
ROBERTS, NJ .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1983, 128 (03) :516-522
[7]   ANTIGEN PROCESSING AND PRESENTATION TO T-CELLS [J].
GREY, HM ;
CHESNUT, R .
IMMUNOLOGY TODAY, 1985, 6 (03) :101-106
[8]   CHANGES IN PHOSPHATIDYLGLYCEROL IN BRONCHOALVEOLAR LAVAGE FLUIDS FROM PATIENTS WITH CRYPTOGENIC FIBROSING ALVEOLITIS [J].
HUGHES, DA ;
HASLAM, PL .
CHEST, 1989, 95 (01) :82-89
[9]  
HUNIG T, 1983, EUR J IMMUNOL, V13, P596, DOI 10.1002/eji.1830130716
[10]   SURFACTANT FOR THE TREATMENT OF RESPIRATORY-DISTRESS SYNDROME [J].
JOBE, A ;
IKEGAMI, M .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1987, 136 (05) :1256-1275