ELEVATED ACTIVITIES OF PROTEIN-KINASE-C AND TYROSINE KINASE CORRELATE TO LEUKEMIC-CELL AGGRESSIVENESS

被引:11
作者
AFLALO, E
WOLFSON, M
OFIR, R
WEINSTEIN, Y
机构
[1] Department of Microbiology and Immunology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva
关键词
D O I
10.1002/ijc.2910500127
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We report a linkage between cell aggressiveness, protein kinase C (PKC) activity, tyrosine kinase (PTK) activity and serum requirement. We used 2 leukemic cell lines induced by Moloney murine leukemia virus (MLV). One line was highly aggressive (BS-24-1) and required low serum concentrations (3%) for optimal growth in comparison to the less aggressive line (RO2T) that needed 10% serum for optimal growth. The more malignant cells exhibited higher PKC and PTK activity. This activity was independent of serum concentration between 0.01-10%. In contrast, the weakly malignant cells need a high serum concentration (100%) for optimal PKC or PTK activity. Immunoblot analysis revealed a higher level of PKC protein in the BS-24-1 cells than in the RO2T cells. Serum induction of PKC activity did not change the amount of PKC protein in the cytosol or the membrane fractions, indicating post-translational mechanism regulation of PKC. We suggest that the aggressiveness of BS-24-1 resulted from its ability to become independent of growth regulation by serum factors, via autocrine stimulation of PKC and PTK.
引用
收藏
页码:136 / 141
页数:6
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