SOLID-PHASE EXTRACTION OF PROSTANOIDS USING AN AUTOMATIC SAMPLE PREPARATION SYSTEM

被引：4

作者：

HOTTER, G

GOMEZ, G

RAMIS, I

BIOQUE, G

ROSELLOCATAFAU, J

GELPI, E

机构：

[1] Department of Neunochemistry, Eicosanoid Branch, Centro de Investigación y Desarrollo, 08034 Barcelona

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 607卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)87081-I

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A commercial automated solid-phase extraction system for cyclooxygenase arachidonic acid metabolites in urine samples has been evaluated. Comparison of manual and automatic batch (36 samples) extraction procedures for tritium labelled prostanoids added as tracers to urine samples has shown equivalent results with recoveries greater than 90% for prostaglandins E2, F2-alpha and 6-keto prostaglandin F1-alpha as well as thromboxane B2. Analyte stability is not affected by the automated procedure, which uses less solvents and has a faster overall processing time than the manual method. The automated system has been applied to the extraction of prostanoids in urine samples from workers exposed to dichloroethane.

引用

页码：239 / 243

页数：5

共 11 条

[1] HIGH-PERFORMANCE (PRESSURE) LIQUID-CHROMATOGRAPHY SEPARATION AND QUANTIFICATION OF PICOMOLE AMOUNTS OF PROSTAGLANDINS UTILIZING A NOVEL TRIETHYLAMINE FORMATE BUFFER [J].

DESIDERIO, DM ;

CUNNINGHAM, MD ;

TRIMBLE, JA .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1981, 4 (07) :1261-1268

[2] MODERN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC-RADIOIMMUNOASSAY STRATEGIES FOR THE STUDY OF EICOSANOIDS IN BIOLOGICAL SAMPLES [J].

GELPI, E ;

RAMIS, I ;

HOTTER, G ;

BIOQUE, G ;

BULBENA, O ;

ROSELLO, J .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 492 :223-250

[3]

Green K, 1986, Adv Prostaglandin Thromboxane Leukot Res, V16, P309

[4]

GREEN K, 1991, ADV PROSTAGLANDIN TH, V41, P529

[5] ALTERED LEVELS OF TISSUE AND URINARY PROSTACYCLIN IN RATS SUBJECTED TO PANCREAS TRANSPLANTATION [J].

HOTTER, G ;

CATAFAU, JR ;

ARTIGOT, M ;

BULBENA, O ;

PI, F ;

SAENZ, A ;

CRUZ, LF ;

GELPI, E .

PROSTAGLANDINS, 1991, 41 (06) :529-536

[6] INCREASED RENAL TXA2 SYNTHESIS IN DIABETES-MELLITUS - SIMULTANEOUS DETERMINATION OF URINARY TXB2 AND 2,3-DINOR-TXB2 [J].

KATAYAMA, S ;

INABA, M ;

MARUNO, Y ;

OMOTO, A ;

KAWAZU, S ;

ISHII, J ;

SAWADA, M .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1990, 39 (01) :47-51

[7] FULLY AUTOMATED SAMPLE HANDLING-SYSTEM FOR LIQUID-CHROMATOGRAPHY BASED ON PRECOLUMN TECHNOLOGY AND AUTOMATED CARTRIDGE EXCHANGE [J].

NIELEN, MWF ;

VALK, AJ ;

FREI, RW ;

BRINKMAN, UAT ;

MUSSCHE, P ;

DENIJS, R ;

OOMS, B ;

SMINK, W .

JOURNAL OF CHROMATOGRAPHY, 1987, 393 (01) :69-83

[8] RAPID EXTRACTION OF OXYGENATED METABOLITES OF ARACHIDONIC-ACID FROM BIOLOGICAL SAMPLES USING OCTADECYLSILYL SILICA [J].

POWELL, WS .

PROSTAGLANDINS, 1980, 20 (05) :947-957

[9] SIMULTANEOUS REVERSED-PHASE EXTRACTION OF LIPOXYGENASE AND CYCLOOXYGENASE METABOLITES OF ARACHIDONIC-ACID IN NASAL SECRETIONS - METHODOLOGICAL ASPECTS [J].

RAMIS, I ;

ROSELLOCATAFAU, J ;

ARTIGOT, M ;

BULBENA, O ;

PICADO, C ;

GELPI, E .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 532 (02) :217-225

[10]

VONEULER VS, 1988, PROSTAGLANDINS BIOL, P1

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