VISUALIZATION OF SECALE-CEREALE DNA IN WHEAT-GERM PLASM BY FLUORESCENT IN-SITU HYBRIDIZATION

被引：43

作者：

ISLAMFARIDI, MN ^{[1
]}

MUJEEBKAZI, A ^{[1
]}

机构：

[1] CIMMYT,MEXICO CITY 06600,DF,MEXICO

来源：

THEORETICAL AND APPLIED GENETICS | 1995年 / 90卷 / 05期

关键词：

ALIEN CHROMOSOME ADDITIONS; FLUORESCENT IN SITU HYBRIDIZATION; GENOMIC DNA; INTERGENERIC HYBRIDS; WHEAT RYE TRANSLOCATIONS;

D O I：

10.1007/BF00222120

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Homozygous wheat/rye (1BL/1RS or 1AS/1RL) translocation lines have significantly contributed to wheat production, and several other wheat/rye translocation lines show a potential promise against biotic and abiotic stresses. Detecting the presence of rye at the chromosome level is feasible by C-banding and isozyme protocols, but the diagnostic strength of genomic in situ hybridization for eventually analyzing smaller DNA introgressions has greater significance. As a first step we have applied the genomic in situ hybridization technique to detect rye chromosomes in a wheat background using germ plasm of agricultural significance. By this method rye contributions to the translocations 1BL/1RS, 1AL/1RS, 5AS/5RL and 6BS/6RL could be identified. Differential labelling has further enabled the detection of rye and Thinopyrum bessarabicum chromosomes in a trigeneric hybrid of Triticum aestivum/Th. bessarabicum//Secale cereale.

引用

页码：595 / 600

页数：6

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