STRUCTURE AND ORGANIZATION OF THE GAS VESICLE GENE-CLUSTER ON THE HALOBACTERIUM-HALOBIUM PLASMID-PNRC100

被引:49
作者
JONES, JG [1 ]
YOUNG, DC [1 ]
DASSARMA, S [1 ]
机构
[1] UNIV MASSACHUSETTS,DEPT MICROBIOL,AMHERST,MA 01003
关键词
GAS VACUOLES; NUCLEOTIDE SEQUENCE; MULTICOPY GENE; PROTEIN REPEATS; INSERTION SEQUENCES; TRANSPOSITION; ARCHAEBACTERIA;
D O I
10.1016/0378-1119(91)90549-Q
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Halobacterium halobium strain NRC-1 contains intracellular gas-filled vesicles (GVs) that confer buoyancy to the cells. Cloning of the major GV protein (GvpA)-encoding gene, gvpA, and analysis of GV-deficient mutants (Vac-) of H. halobium led to the identification of a region of a 200-kb plasmid, pNRC100, important for GV synthesis. We report here the nucleotide sequence of an 8520-bp region which, including gvpA, contains twelve open reading frames (ORFs) that are organized into two divergent transcription units, gvpAC oriented rightward, and gvpD, E, F, G, H, I, J, K, L, and M located upstream from gvpAC and oriented leftward. Insertions into the gvpA promoter and gvpD and E resulted in the Vac- phenotype. The overall gene organization is highly compact with the end of one ORF overlapping with the beginning of the next in most cases. The gene cluster is bracketed by two ISH8 element copies in inverted orientation, an organization suggestive of a composite transposon. Comparison of predicted amino acid sequences showed homology between GvpA, and the gvpJ and gvpM putative gene products. The putative gvpC gene product contains eight copies of an imperfectly repeated sequence with similarity to repeats in a cyanobacterial GvpC plus a highly acidic C-terminal region not found in the cyanobacterial homologue.
引用
收藏
页码:117 / 122
页数:6
相关论文
共 29 条
[1]   COMPARATIVE STUDY OF STRUCTURE OF GAS VACUOLES [J].
COHENBAZIRE, G ;
KUNISAWA, R ;
PFENNIG, N .
JOURNAL OF BACTERIOLOGY, 1969, 100 (02) :1049-+
[2]   A DEVELOPMENTALLY REGULATED GVPABC OPERON IS INVOLVED IN THE FORMATION OF GAS VESICLES IN THE CYANOBACTERIUM CALOTHRIX-7601 [J].
DAMERVAL, T ;
HOUMARD, J ;
GUGLIELMI, G ;
CSISZAR, K ;
DEMARSAC, NT .
GENE, 1987, 54 (01) :83-92
[3]   HIGH-FREQUENCY MUTATIONS IN A PLASMID-ENCODED GAS VESICLE GENE IN HALOBACTERIUM-HALOBIUM [J].
DASSARMA, S ;
HALLADAY, JT ;
JONES, JG ;
DONOVAN, JW ;
GIANNASCA, PJ ;
DEMARSAC, NT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6861-6865
[5]   A PLASMID-ENCODED GAS VESICLE PROTEIN GENE IN A HALOPHILIC ARCHAEBACTERIUM [J].
DASSARMA, S ;
DAMERVAL, T ;
JONES, JG ;
DEMARSAC, NT .
MOLECULAR MICROBIOLOGY, 1987, 1 (03) :365-370
[6]   MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF A DEVELOPMENTALLY REGULATED GENE FROM THE CYANOBACTERIUM CALOTHRIX-PCC-7601 - A GAS VESICLE PROTEIN GENE [J].
DEMARSAC, NT ;
MAZEL, D ;
BRYANT, DA ;
HOUMARD, J .
NUCLEIC ACIDS RESEARCH, 1985, 13 (20) :7223-7236
[7]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395
[8]  
Dundas I E, 1977, Adv Microb Physiol, V15, P85, DOI 10.1016/S0065-2911(08)60315-X
[9]   THE BACTERIORHODOPSIN GENE [J].
DUNN, R ;
MCCOY, J ;
SIMSEK, M ;
MAJUMDAR, A ;
CHANG, SH ;
RAJBHANDARY, UL ;
KHORANA, HG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (11) :6744-6748
[10]  
GERL L, 1988, J BIOL CHEM, V263, P13246