MYROSINASE IS LOCALIZED TO THE INTERIOR OF MYROSIN GRAINS AND IS NOT ASSOCIATED TO THE SURROUNDING TONOPLAST MEMBRANE

被引：49

作者：

HOGLUND, AS

LENMAN, M

RASK, L

机构：

[1] Department of Cell Research, Swedish University of Agricultural Sciences, S-75007 Uppsala

来源：

PLANT SCIENCE | 1992年 / 85卷 / 02期

关键词：

SINAPIS-ALBA; MYROSINASE; MYROSIN GRAINS; IMMUNOGOLD LOCALIZATION BY MONOCLONAL ANTIBODIES;

D O I：

10.1016/0168-9452(92)90112-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The distribution of myrosinase was investigated in embryos of different age of Sinapis alba by immuno electron microscopy. Using a well characterized monoclonal antibody the myrosinase was found to be exclusively localized to the interior of the myrosin grains, a finding in contrast to earlier studies by light microscopy which indicated either a cytoplasmic distribution or an association to the tonoplast membrane surrounding the myrosin grains.

引用

页码：165 / 170

页数：6

共 19 条

[1] PURIFICATION, CHARACTERIZATION AND PARTIAL AMINO-ACID SEQUENCING OF BETA-THIOGLUCOSIDASE FROM BRASSICA-NAPUS L [J].