MODELING THE 3-D RNA DISTRIBUTION IN THE BALBIANI RING GRANULE

被引:12
作者
OLINS, AL
OLINS, DE
OLMAN, V
LEVY, HA
BAZETTJONES, DP
机构
[1] OAK RIDGE NATL LAB, DIV BIOL, OAK RIDGE, TN 37831 USA
[2] UNIV CALGARY, DEPT MED BIOCHEM, CALGARY T2N 4N1, AB, CANADA
关键词
D O I
10.1007/BF00417877
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mature Balbiani Ring (BR) granules in situ were stained with the nucleic acid specific stain, osmium ammine-B, recorded by electron spectroscopic imaging and reconstructed by electron microscope tomography to examine the three-dimensional (3-D) distribution of BR heterogeneous nuclear RNA (hnRNA). The BR2 gran ules contain ca. 37 kb of mRNA. Reconstructed BR granules were selected to emphasize one of the prevalent conformations seen in the sectioned salivary glands, the en face or ''pin-wheel'' conformation. A variety of image processing and volume-rendering operations were applied to the set of reconstructed BR granules. Some of the conclusions of this study are the following: (1) RNA distribution is not uniform throughout the granule; (2) RNA is condensed into about ten particles per granule, which all appear to possess approximately the same RNA stain density; (3) heterogeneity exists in the positions and sizes of particles within the various BR granules. These data argue for the folding of a beaded ribbon, consisting of connected particulate condensations of BR mRNA, possessing considerable 3-D flexibility, even in the packaged state. A comparison of this beaded-ribbon model and a prior folded hnRNP fiber model is also presented.
引用
收藏
页码:302 / 310
页数:9
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