SINGLE STEP OXIDATIVE BINDING OF ANTIBODIES TO HYDRAZIDE-MODIFIED EUPERGIT-C

被引：5

作者：

FLEMINGER, G

SOLOMON, B

WOLF, T

HADAS, E

机构：

[1] Department of Biotechnology, Faculty of Life Sciences, Tel-Auiu university, Tel-Aviv

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1990年 / 26卷 / 03期

关键词：

carbohydrates; Eupergit C; Monoclonal antibodies; periodate oxidation;

D O I：

10.1007/BF02921504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A single step periodate oxidative binding of glycoproteins to synthetic polymeric matrices is proposed. By this procedure, the glycoprotein to be immobilized is simultaneously mixed with sodium periodate and a hydrazide-containing matrix. The carbohydrate moieties of the protein are oxidized and react with matrix. The single step reaction is faster than the commonly-used multistep procedure and it allows conjugation of minute amounts of glycoproteins to the matrix. © 1990 Humana Press Inc.

引用

页码：231 / 238

页数：8

共 6 条

[1]

ENGVALL E, 1972, J IMMUNOL, V109, P129

[2] ORIENTED IMMOBILIZATION OF PERIODATE-OXIDIZED MONOCLONAL-ANTIBODIES ON AMINO AND HYDRAZIDE DERIVATIVES OF EUPERGIT-C [J].