VERAPAMIL ENHANCES HIGH-DENSITY-LIPOPROTEIN PROCESSING IN HEP G2 CELLS PRELOADED WITH CHOLESTEROL

被引：1

作者：

CHAPPEYGILLET, B ^{[1
]}

SALMON, S ^{[1
]}

MAZIERE, C ^{[1
]}

AUCLAIR, M ^{[1
]}

MAZIERE, JC ^{[1
]}

机构：

[1] UNIV PARIS 06,BIOCHIM LAB,27 RUE CHALIGNY,F-75571 PARIS 12,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1052卷 / 02期

关键词：

(Human hepatocyte); Cholesterol; HDL; Verapamil;

D O I：

10.1016/0167-4889(90)90221-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the calcium channel blocker of the arylalkylamine series verapamil have been investigated on high-density lipoprotein (HDL3) catabolism in the human hepatoma cell line Hep G2. It was found that verapamil markedly enhanced HDL3 binding, uptake and degradation in Hep G2 cells preloaded with nonlipoprotein cholesterol. This effect was dose-dependent, and a 1.5-2-fold increase of the three studied parameters was observed in cells pretreated 24 h with 100 μM verapamil. No significant effect of the drug was found in cells not preincubated with cholesterol. Verapamil induced an increase in the cellular cholesterol content in preloaded cells. Other calcium antagonists such as diltiazem, nifedipine, nitrendipine or amphiphilic drugs such as phenothiazines and propranolol also enhanced HDL3 uptake by Hep G2 cells. These effects of verapamil on HDL3 metabolism could be related to its amphiphilic characteristics, and to its calcium antagonist properties. © 1990.

引用

页码：273 / 277

页数：5

共 24 条

[11]

MAYATOSHI ED, 1980, BIOCHEMISTRY-US, V19, P3414

[12] RAPID ANALYSIS OF CELLULAR LIPIDS WITHOUT EXTRACTION [J].

MAZIERE, C ;

MAZIERE, JC ;

MORA, L ;

POLONOVSKI, J .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1987, 14 (05) :267-272

[13] EFFECTS OF THE CALCIUM IONOPHORE A-23187 ON LOW-DENSITY LIPOPROTEIN PROCESSING AND LIPID-METABOLISM IN CULTURED HUMAN-FIBROBLASTS [J].

MAZIERE, JC ;

MAZIERE, C ;

MORA, L ;

GARDETTE, J ;

POLONOVSKI, J .

FEBS LETTERS, 1984, 177 (01) :76-80

[14] EFFECT OF CYCLIC-AMP ON LOW-DENSITY LIPOPROTEIN BINDING AND INTERNALIZATION BY CULTURED HUMAN-FIBROBLASTS [J].

MAZIERE, JC ;

MAZIERE, C ;

GARDETTE, J ;

MORA, L ;

POLONOVSKI, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 112 (03) :795-800

[15]

MAZIERE JC, 1983, FEBS LETT, V1632, P396

[16] REGULATION OF HIGH-DENSITY LIPOPROTEIN RECEPTOR ACTIVITY IN CULTURED HUMAN-SKIN FIBROBLASTS AND HUMAN ARTERIAL SMOOTH-MUSCLE CELLS [J].

ORAM, JF ;

BRINTON, EA ;

BIERMAN, EL .

JOURNAL OF CLINICAL INVESTIGATION, 1983, 72 (05) :1611-1621

[17]

PAOLETTI R, 1988, BIOTECHNOLOGY CLIN M, P45

[18]

POLONOVSKI J, 1986, LIPID METABOLISM ITS, P19

[19]

RANGHANATAN S, 1981, BIOCHEM BIOPH RES CO, V107, P217

[20] VERAPAMIL SUPPRESSES ATHEROSCLEROSIS IN CHOLESTEROL-FED RABBITS [J].

ROULEAU, JL ;

PARMLEY, WW ;

STEVENS, J ;

WIKMANCOFFELT, J ;

SIEVERS, R ;

MAHLEY, RW ;

HAVEL, RJ ;

BRECHT, W .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 1983, 1 (06) :1453-1460

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