A MICROCOMPUTER-BASED PROGRAM FOR VIDEO ANALYSIS OF CHEMOTAXIS UNDER AGAROSE

被引：13

作者：

COATES, TD ^{[1
]}

HARMAN, JT ^{[1
]}

MCGUIRE, WA ^{[1
]}

机构：

[1] INDIANA UNIV,JAMES WHITCOMB RILEY HOSP CHILDREN,SCH MED,DEPT PEDIAT,DIV PEDIAT HEMATOL ONCOL,INDIANAPOLIS,IN 46223

来源：

COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE | 1985年 / 21卷 / 03期

关键词：

Bacteriology; -; COMPUTERS; MICROCOMPUTER - Medical Applications - IMAGE PROCESSING - Image Analysis - MICROSCOPIC EXAMINATION - Medical Applications;

D O I：

10.1016/0169-2607(85)90004-5

中图分类号：

TP39 [计算机的应用];

学科分类号：

081203 ; 0835 ;

摘要：

The polymorphonuclear neutrophil (PMN) is the primary cell in host defense against bacterial invasion. This cell senses a bacterium, moves to the site of infection, and then ingests and kills the bacterium. The process of cell movement may be separated into two basic components: the ability to undergo directed movement, or chemotaxis, and the regulation of velocity of movement, or chemokinesis. We have developed a computerized system for the quantification of video images of cell migration patterns obtained in the chemotaxis under agarose assay. This system allows either manual or automatic measurement of standard parameters of chemotaxis, as well as quantification of three new parameters, which reflect mean cell movement and chemokinesis. Our system operates on an inexpensive microcomputer, is easy to use and provides data output in a format that is directly transferable to standard statistical packages.

引用

页码：195 / 202

页数：8