RELATIONSHIP BETWEEN STRUCTURE, CONFORMATIONAL FLEXIBILITY, AND BIOLOGICAL-ACTIVITY OF AGONISTS AND ANTAGONISTS AT THE N-METHYL-D-ASPARTIC ACID SUBTYPE OF EXCITATORY AMINO-ACID RECEPTORS

The relationship between conformational flexibility and agonist or antagonist actions at the N-Methyl-D-aspartic acid (NMDA) subtype of central L-glutamic acid (GLU) receptors of a series of racemic piperidinedicarboxylic acids (PDAs) was studied. The conformational analyses were based on 1H NMR spectroscopy and supported by computer simulations and molecular mechanics calculations. While the trans forms of 2, 3-PDA and 2, 4-PDA and ci.s-2, 5-PDA show NMDA receptor agonist activities, cis-2, 3-PDA and cis-2,4-PDA are NMDA antagonists. The compounds trans-2,5-PDA and cis-2,6-PDA did not interact with NMDA receptors. Each of the three cyclic acidic amino acids showing NMDA agonist activities was found to exist as an equilibrium mixture of two conformers in aqueous solution. In contrast, the NMDA antagonists cis-2,3-PDA and cis-2,4-PDA as well as the inactive compounds trans-2,5-PDA and cis-2,6-PDA were shown to exist predominantly in a single conformation. These results seem to indicate that a certain degree of conformational flexibility of analogues of GLU is a prerequisite for activation of, but not for binding to, the NMDA receptor. © 1990, American Chemical Society. All rights reserved.