DOES DAPI DETECT CYTOPLASMIC DNA

被引：4

作者：

JOHANSEN, B ^{[1
]}

SEBERG, O ^{[1
]}

PETERSEN, G ^{[1
]}

ARCTANDER, P ^{[1
]}

机构：

[1] UNIV COPENHAGEN, INST ZOOL, DEPT POPULAT BIOL, DK-2100 COPENHAGEN O, DENMARK

来源：

AMERICAN JOURNAL OF BOTANY | 1995年 / 82卷 / 10期

关键词：

D O I：

10.2307/2446243

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ultrastructural observations have revealed that plastids are present in orchid pollen tubes, but the DNA-binding fluorochrome 4',6-diamidino-2-phenylindole (DAPI) does not localize any DNA in the pollen tube plastids at optimum binding and fluorescence conditions. However, the plastids do contain DNA since the gene coding for the large subunit of rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase, rbcL) has been amplified by the polymerase chain reaction from orchid pollen tubes. It is therefore concluded that DAPI is an unreliable fluorochrome for detecting plastid DNA.

引用

页码：1215 / 1219

页数：5

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