RAS INDUCES A GENERAL DNA DEMETHYLATION ACTIVITY IN MOUSE EMBRYONAL P19 CELLS

被引：48

作者：

SZYF, M

THEBERGE, J

BOZOVIC, V

机构：

[1] Dept. of Pharmacol. and Therapeutics, McGill University, Montreal

[2] Dept. of Pharmacol. and Therapeutics, McGill University, Montreal, Que. H3G 1Y6

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 21期

关键词：

D O I：

10.1074/jbc.270.21.12690

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate that expression of v-Ha-ras in mouse embryonal P19 cells results in genome-wide demethylation. Analysis of the pattern of methylation of specific genes reveals that different types of genes are demethylated in the ras transfectants: skeletal muscle specific genes, a gene specifically expressed in the adrenal cortex (c21), ubiquitous genes, and exogenously introduced sequences. Transient transfection and in vitro demethylation assays reveal that the ras transfectants express high levels of a general DNA demethylation activity. This demonstrates that the general DNA demethylation activity in mouse embryonal cells is controlled by an important cellular signal transducer and that DNA demethylase is a potential downstream effector of Ras.

引用

页码：12690 / 12696

页数：7

共 32 条

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